Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes.

Abstract

PURPOSE:

GOLPH3 has been shown to be involved in glioma proliferation. In this study, we aimed to demonstrate that GOLPH3 can serve as a target for glioma gene therapy.

METHODS:

During the experiment, cationic liposomes with angiopep-2 (A2-CL) were used to deliver siGOLPH3 crossing the blood-brain barrier and reaching the glioma.

RESULTS:

At the cellular level, the A2-CL/siGOLPH3 could silence GOLPH3 and then effectively inhibited the proliferation of cells. In vivo experiments, using U87-GFP-Luci-bearing BALB/c mouse models, we demonstrated that A2-CL could deliver GOLPH3-siRNA specifically to glioma and effectively inhibit glioma growth.

CONCLUSIONS:

This study shows that GOLPH3 has great potential as a target for the gene therapy of glioma and is of great value in precise medical applications.

• Authors: Zixuan Yuan, Liang Zhao, Yafei Zhang, Shun Li, Bomin Pan, Lei Hua, Zhen Wang, Chengkun Ye, Jun Lu, Rutong Yu, Hongmei Liu
• Journal: Journal of neuro-oncology (J Neurooncol) Vol. 140 Issue 2 Pg. 249-260 (Nov 2018) ISSN: 1573-7373 [Electronic] United States
• PMID: 30105446 (Publication Type: Journal Article)

Chemical References

• Cations
• GOLPH3 protein, human
• Liposomes
• Membrane Proteins
• RNA, Small Interfering

Topics

• Animals
• Brain (diagnostic imaging, metabolism, pathology)
• Brain Neoplasms (diagnostic imaging, metabolism, pathology, therapy)
• Cations (chemistry, pharmacokinetics, therapeutic use)
• Cell Line
• Genetic Therapy
• Glioma (diagnostic imaging, metabolism, pathology, therapy)
• Humans
• Liposomes (chemistry, pharmacokinetics, therapeutic use)
• Male
• Membrane Proteins (antagonists & inhibitors, genetics)
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• RNA Interference
• RNA, Small Interfering (administration & dosage, pharmacokinetics)