The N-terminal polypeptide derived from vMIP-II exerts its anti-tumor activity in human breast cancer by regulating lncRNA SPRY4-IT1.

Abstract

Accumulating evidence demonstrates that long non-coding RNA (lncRNA) sprouty4-intron transcript 1 (lncRNA SPRY4-IT1) plays a vital role in the development of breast cancer. However, the underlying mechanism has not been eventually illuminated. We aimed to explore the biological activity of lncRNA SPRY4-IT1 in breast cancer cells and whether N-terminal polypeptide derived from viral macrophage inflammatory protein II (NT21MP) could exert its anti-tumor effect by regulating lncRNA SPRY4-IT1 and its target gene SKA2 Real-time RT-PCR, Western blotting, wound healing, and invasion assays were used to achieve this goal. We found that lncRNA SPRY4-IT1 was highly expressed in breast cancer cells. Moreover, NT21MP markedly inhibited biological effects of breast cancer cells by regulating lncRNA SPRY4-IT1, which was partially achieved through SKA2. Our findings suggested that lncRNA SPRY4-IT1 could serve as a novel biomarker by NT21MP for breast cancer.

• Authors: Haihua Wu, Yueyue Wang, Tiantian Chen, Yu Li, Haifeng Wang, Lingyu Zhang, Sulian Chen, Wenrui Wang, Qingling Yang, Changjie Chen
• Journal: Bioscience reports (Biosci Rep) Vol. 38 Issue 5 (10 31 2018) ISSN: 1573-4935 [Electronic] England
• PMID: 30104400 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The Author(s).

Chemical References

• Biomarkers, Tumor
• Chemokines
• Chromosomal Proteins, Non-Histone
• RNA, Long Noncoding
• SKA2 protein, human
• long noncoding RNA SPRY4-IT1, human
• vMIP-II

Topics

• Biomarkers, Tumor (genetics)
• Breast Neoplasms (drug therapy, genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chemokines (genetics, pharmacology)
• Chromosomal Proteins, Non-Histone (genetics)
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Herpesviridae (chemistry, genetics)
• Humans
• RNA, Long Noncoding (genetics)