Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle.

Abstract

Optical imaging technologies improve clinical diagnostic accuracy of early gastric cancer (EGC). However, there was a lack of imaging agents exhibiting molecular specificity for EGCs. Here, we employed the dye labeled human heavy-chain ferritin (HFn) as imaging nanoprobe, which recognizes tumor biomarker transferrin receptor 1 (TfR1), to enable specific EGC imaging using confocal laser endomicroscopy (CLE). TfR1 expression was initially examined in vitro in gastric tumor cells and in vivo through whole-body fluorescence and CLE imaging in tumor-bearing mice. Subsequently, dye labeled HFn was topically applied to resected human tissues for EGC detection. CLE analysis of TfR1-targeted fluorescence imaging allowed distinction of neoplastic from non-neoplastic tissues (P < 0.0001), and TfR1 expression level was found to correlate with EGC differentiation degrees (P < 0.0001). Notably, the CLE evaluation correlated well with the immunohistochemical findings (κ-coefficient: 0.8023). Our HFn-nanoprobe-based CLE increases the accuracy of EGC detection and enables visualization of tumor margins and endoscopic resection.

• Authors: Yang Du, Kelong Fan, Hejun Zhang, Li Li, Peixia Wang, Jiuyang He, Shigang Ding, Xiyun Yan, Jie Tian
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 14 Issue 7 Pg. 2259-2270 (10 2018) ISSN: 1549-9642 [Electronic] United States
• PMID: 30056091 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Antigens, CD
• CD71 antigen
• Fluorescent Dyes
• Receptors, Transferrin
• Apoferritins

Topics

• Aged
• Aged, 80 and over
• Animals
• Antigens, CD (metabolism)
• Apoferritins (administration & dosage, chemistry, metabolism)
• Endoscopy (methods)
• Female
• Fluorescent Dyes (chemistry)
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Microscopy, Fluorescence
• Middle Aged
• Molecular Imaging (methods)
• Nanoparticles (administration & dosage, chemistry)
• Prognosis
• Receptors, Transferrin (metabolism)
• Stomach Neoplasms (diagnostic imaging, metabolism, pathology)
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays