Evaluation of IP-10 in Quantiferon-Plus as biomarker for the diagnosis of latent tuberculosis infection.

Abstract

The QuantiFERON-TB Gold Plus (QFT-Plus) is a new test for latent tuberculosis infection (LTBI) diagnosis, in which has been added a new tube containing shorter peptides stimulating CD8 T-cells and CD4-stimulating-peptides. Measurement of alternative biomarkers to Interferon-γ (IFN-γ) in QFT-Plus may improve its sensitivity. Interferon-γ inducible protein 10 (IP-10), has been proposed as a tuberculosis (TB) biomarker. We aimed to evaluate the IP-10 accuracy in QFT-Plus for LTBI diagnosis. QFT-Plus was performed in 36 active TB, 31 LTBI and 16 healthy donors (HD). IP-10 was detected by ELISA. IP-10 is increased in TB1 and TB2 tubes in subjects with active TB and LTBI compared to HD. A ROC analysis comparing active TB and HD was performed and a cut-off of 1174 pg/mL for TB1 and 928.8 pg/mL for TB2 identified active TB with 86% sensitivity (Se) and 94% specificity (Sp). Moreover, increased IP-10 in response to TB1 was found in subjects with LTBI compared to those with active TB. A cut-off point of ≥16,108 pg/mL was chosen to maximize the test performance. However, the test predicted LTBI only with 58% Se and 61% Sp. These results suggest that IP-10 is an alternative biomarker to IFN-γ in the QFT-Plus format.

• Authors: Linda Petrone, Valentina Vanini, Teresa Chiacchio, Elisa Petruccioli, Gilda Cuzzi, Vincenzo Schininà, Fabrizio Palmieri, Giuseppe Ippolito, Delia Goletti
• Journal: Tuberculosis (Edinburgh, Scotland) (Tuberculosis (Edinb)) Vol. 111 Pg. 147-153 (07 2018) ISSN: 1873-281X [Electronic] Scotland
• PMID: 30029901 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018. Published by Elsevier Ltd.

Chemical References

• Biomarkers
• CXCL10 protein, human
• Chemokine CXCL10
• IFNG protein, human
• Interferon-gamma

Topics

• Adult
• Bacterial Load
• Biomarkers (blood)
• Case-Control Studies
• Chemokine CXCL10 (blood, immunology)
• Enzyme-Linked Immunospot Assay (methods)
• Female
• Host-Pathogen Interactions
• Humans
• Interferon-gamma (blood, immunology)
• Interferon-gamma Release Tests (methods)
• Latent Tuberculosis (blood, diagnosis, immunology, microbiology)
• Male
• Middle Aged
• Mycobacterium tuberculosis (immunology, pathogenicity)
• Predictive Value of Tests
• Reproducibility of Results