Effects of estrogen receptor GPR30 agonist G1 on neuronal apoptosis and microglia polarization in traumatic brain injury rats.

Abstract

PURPOSE:

To investigate the effects of estrogen G protein-coupled receptor 30 (GPR30) agonist G1 on hippocampal neuronal apoptosis and microglial polarization in rat traumatic brain injury (TBI).

METHODS:

Male SD rats were randomly divided into sham group, TBI + vehicle group, TBI + G1 group. Experimental moderate TBI was induced using Feeney's weigh-drop method. G1 (100μg/kg) or vehicle was intravenously injected from femoral vein at 30 min post-injury. Rats were sacrificed at 24 h after injury for detection of neuronal apoptosis and microglia polarization. Neuronal apoptosis was assayed by immunofluorescent staining of active caspase-3. M1 type microglia markers (iNOS and IL-1β) and M2 type markers (Arg1 and IL-4) were examined by immunoblotting or ELISA. Total protein level of Akt and phosphorylated Akt were assayed by immunoblotting.

RESULTS:

G1 significantly reduced active caspase-3 positive neurons in hippocampus. Meanwhile G1 increased the ratio of Arg1/iNOS. IL-1β production was decreased but IL-4 was increased after G1 treatment. G1 treatment also increased the active form of Akt.

CONCLUSIONS:

GPR30 agonist G1 inhibited neuronal apoptosis and favored microglia polarization to M2 type.

• Authors: Meng-Xian Pan, Jun-Chun Tang, Rui Liu, Yu-Gong Feng, Qi Wan
• Journal: Chinese journal of traumatology = Zhonghua chuang shang za zhi (Chin J Traumatol) Vol. 21 Issue 4 Pg. 224-228 (Aug 2018) ISSN: 1008-1275 [Print] China
• PMID: 30017543 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Daping Hospital and the Research Institute of Surgery of the Third Military Medical University. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• Gper1 protein, rat
• Interleukin-1beta
• Receptors, G-Protein-Coupled
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Apoptosis (drug effects)
• Brain Injuries, Traumatic (drug therapy, pathology)
• Cell Polarity
• Hippocampus (drug effects)
• Interleukin-1beta (biosynthesis)
• Male
• Microglia (drug effects)
• Neurons (drug effects)
• Proto-Oncogene Proteins c-akt (metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptors, G-Protein-Coupled (agonists)