Apoptotic and antiproliferative effects of Stigmast-5-en-3-ol from Dendronephthya gigantea on human leukemia HL-60 and human breast cancer MCF-7 cells.
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| Abstract |
The genus Dendronephthya encompasses marine soft corals that produce a wide spectrum of biofunctional terpenoids. Anticancer properties of these metabolites are widely exploited as potential chemotherapeutic agents. The present study reports the purification and isolation of a potential antiproliferative constituent, stigmast-5-en-3-ol from the 70% ethanol extract of the soft coral Dendronephthya gigantea. Among several other 3β-hydroxy-Δ5-steroidal congeners, stigmast-5-en-3-ol indicated prominent antiproliferative effects on HL-60 (leukemia) and MCF-7 (breast cancer) cell lines with IC50 values of 37.82 and 45.17 μg/ml respectively. Stigmast-5-en-3-ol increased apoptotic body formation, accumulation of sub G1 apoptotic cells, and DNA damage in HL-60 and MCF-7 cells. It increased the expression of Bax, caspases, and PARP cleavage while decreasing Bcl-xL levels in both cancer cell lines indicating that the effects are arbitrated via the mitochondria-mediated apoptotic pathway. Steroidal derivatives were identified by GC MS/MS and the identity of stigmast-5-en-3-ol was confirmed by NMR spectra. The present study suggests that stigmast-5-en-3-ol could be a promising candidate for anticancer drug research.
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| Authors | I P Shanura Fernando, K K Asanka Sanjeewa, Yong-Seok Ann, Chang-Ik Ko, Seung-Hong Lee, Won Woo Lee, You-Jin Jeon |
| Journal | Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 52 Pg. 297-305 (Oct 2018) ISSN: 1879-3177 [Electronic] England |
| PMID | 30012480 (Publication Type: Journal Article) |
| Copyright | Copyright © 2018 Elsevier Ltd. All rights reserved. |
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