Downregulation of miR-218-5p promotes invasion of oral squamous cell carcinoma cells via activation of CD44-ROCK signaling.

Abstract

The invasion front of oral squamous cell carcinoma (OSCC) harbors the most aggressive cells of the tumor and is critical for cancer invasion and metastasis. MicroRNAs (miRNAs) play important roles in OSCC progression. In this study, we modelled the OSCC invasion front on a microfluidic chip, and investigated differences in miRNA profiles between cells in the invasion front and those in the tumor mass by small RNA sequencing. We found that miR-218-5p was downregulated in invasion front cells and negatively regulates OSCC invasiveness by targeting the CD44-ROCK pathway. Thus, miR-218-5p may serve as a useful therapeutic target for OSCC. Moreover, invasion front cell isolation based-on microfluidic technology provided a useful strategy for cancer invasion study.

• Authors: Xiaojie Li, Jianya He, Miaomiao Shao, Bai Cui, Fei Peng, Jiao Li, Yan Ran, Dong Jin, Jing Kong, Jinming Chang, Liqiang Duan, Xuesong Yang, Yong Luo, Yao Lu, Bingcheng Lin, Tingjiao Liu
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 106 Pg. 646-654 (Oct 2018) ISSN: 1950-6007 [Electronic] France
• PMID: 29990854 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Chemical References

• CD44 protein, human
• Hyaluronan Receptors
• MIRN218 microRNA, human
• MicroRNAs
• rho-Associated Kinases

Topics

• Cell Movement
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Hyaluronan Receptors (metabolism)
• Lab-On-A-Chip Devices
• MicroRNAs (genetics, metabolism)
• Microfluidic Analytical Techniques (instrumentation)
• Mouth Neoplasms (drug therapy, enzymology, genetics, pathology)
• Neoplasm Invasiveness
• Signal Transduction
• Squamous Cell Carcinoma of Head and Neck (drug therapy, enzymology, genetics, pathology)
• Time Factors
• rho-Associated Kinases (metabolism)