Bub1 Facilitates Virus Entry through Endocytosis in a Model of Drosophila Pathogenesis.

Abstract

In order to establish productive infection and dissemination, viruses usually evolve a number of strategies to hijack and/or subvert the host defense systems. However, host factors utilized by the virus to facilitate infection remain poorly characterized. In this work, we found that Drosophila melanogaster deficient in budding uninhibited by benzimidazoles 1 (bub1), a highly conserved subunit of the kinetochore complex regulating chromosome congression (1), became resistant to Drosophila C virus (DCV) infection, evidenced in increased survival rates and reduced viral loads, compared to the wild-type control. Mechanistic analysis further showed that Bub1 also functioned in the cytoplasm and was essentially involved in clathrin-dependent endocytosis of DCV and other pathogens, thus limiting pathogen entry. DCV infection potentially had strengthened the interaction between Bub1 and the clathrin adaptor on the cell membrane. Furthermore, the conserved function of Bub1 was also verified in a mammalian cell line. Thus, our data demonstrated a previously unknown function of Bub1 that could be hijacked by pathogens to facilitate their infection and spread.IMPORTANCE In this work, we identify for the first time that the nuclear protein Bub1 (budding uninhibited by benzimidazoles 1), a highly conserved subunit of the kinetochore complex regulating chromosome congression, has a novel and important function on the cell membrane to facilitate the virus to enter host cells. Bub1 deficiency empowers the host to have the ability to resist viral infection in Drosophila and a human cell line. Bub1 is involved in the virus entry step through regulating endocytosis. The DCV capsid protein can recruit Bub1, and DCV infection can strengthen the interaction between Bub1 and a clathrin-dependent endocytosis component. The restricted entry of vesicular stomatitis virus (VSV) and Listeria monocytogenes in bub1-deficient flies and cell lines was also observed. Therefore, our data implicate a previously unknown function of Bub1 that can be hijacked by pathogens to facilitate their entry, and Bub1 may serve as a potential antiviral therapy target for limiting viral entry.

• Authors: Shuo Yang, Junjing Yu, Zhiqin Fan, Si-Tang Gong, Hong Tang, Lei Pan
• Journal: Journal of virology (J Virol) Vol. 92 Issue 18 (09 15 2018) ISSN: 1098-5514 [Electronic] United States
• PMID: 29976667 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Yang et al.

Chemical References

• 3' Untranslated Regions
• Clathrin
• Bub1 spindle checkpoint protein
• Protein Serine-Threonine Kinases

Topics

• 3' Untranslated Regions (physiology)
• Animals
• Clathrin (metabolism)
• Dicistroviridae (genetics, pathogenicity, physiology)
• Drosophila melanogaster (genetics, metabolism, virology)
• Endocytosis
• HEK293 Cells
• Host-Pathogen Interactions (genetics)
• Humans
• Listeria monocytogenes (pathogenicity, physiology)
• Protein Serine-Threonine Kinases (deficiency, genetics)
• Vesicular stomatitis Indiana virus (physiology)
• Viral Load
• Virus Internalization