Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy.

Abstract

BACKGROUND/AIM:

Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro.

MATERIALS AND METHODS:

To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis.

RESULTS:

The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol.

CONCLUSION:

Vδ2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.

• Authors: Yuki Abe, Hirohito Kobayashi, Yoshika Akizawa, Ken Ishitani, Kazunori Hashimoto, Hideo Matsui
• Journal: Anticancer research (Anticancer Res) Vol. 38 Issue 7 Pg. 4327-4331 (Jul 2018) ISSN: 1791-7530 [Electronic] Greece
• PMID: 29970569 (Publication Type: Journal Article)
• Copyright: Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Receptors, Antigen, T-Cell, gamma-delta

Topics

• Adult
• Aged
• Aged, 80 and over
• Ascites (immunology)
• Ascitic Fluid (immunology)
• Female
• Humans
• Immunotherapy, Adoptive (methods)
• Lymphocytes, Tumor-Infiltrating (immunology)
• Middle Aged
• Neoplasms (immunology)
• Receptors, Antigen, T-Cell, gamma-delta
• T-Lymphocytes (immunology)