Abstract | BACKGROUND:
Bladder cancer diagnosis and surveillance includes cystoscopy and cytology. New methods for the detection of bladder cancer are needed, because cystoscopy is invasive and expensive, and because urine cytology is not sensitive enough. OBJECTIVES: The aim of the study was to select potential plasma protein markers for bladder cancer which could be useful in developing a specific laboratory test to improve diagnosis and to establish treatment strategies in order to prevent the recurrence of the disease. MATERIAL AND METHODS: Plasma proteome maps were prepared based on 2-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined with image gel analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of plasma samples from patients with urothelial bladder cancer, and they were compared to normal samples. RESULTS: CONCLUSIONS: The data indicated potential plasma biomarkers associated with inflammation, immunity and coagulation processes accompanying bladder cancer. They could be used for the development of a laboratory test(s) useful in clinical practice.
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Authors | Anna Lemańska-Perek, Jolanta Lis-Kuberka, Adam Lepczyński, Alicja Dratwa-Chałupnik, Krzysztof Tupikowski, Iwona Kątnik-Prastowska, Małgorzata Ożgo |
Journal | Advances in clinical and experimental medicine : official organ Wroclaw Medical University
(Adv Clin Exp Med)
Vol. 28
Issue 3
Pg. 339-346
(Mar 2019)
ISSN: 1899-5276 [Print] Poland |
PMID | 29963784
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Biomarkers, Tumor
- Blood Proteins
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Topics |
- Biomarkers
- Biomarkers, Tumor
(blood)
- Blood Proteins
(analysis)
- Electrophoresis, Gel, Two-Dimensional
- Humans
- Neoplasm Recurrence, Local
- Pilot Projects
- Proteomics
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Two-Dimensional Difference Gel Electrophoresis
- Urinary Bladder Neoplasms
(blood)
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