Abstract |
Platelets contribute to inflammation and their activation has been suggested as versatile effectors of sepsis. Activation of platelets promotes secretion of CD40L that induces sepsis and multiple organ dysfunction syndrome ( MODS). However, the mechanisms regulate platelet-derived CD40L are not fully understood. Activation of PI3K/Akt pathway has been reported as a key component of sepsis, whereas the role of PI3K/Akt pathway in platelet-derived CD40L is unknown. In this study, we identified PI3K/Akt pathway as a key regulator of CD40L secretion by platelets. Significantly, inhibition of PI3K/Akt pathway by Ly294002 attenuated platelet activation and CD40L production. Moreover, PI3K/Akt pathway blocking suppresses vascular endothelial cells in vivo. Furthermore, the expression of biomarkers that represent the severity of sepsis, such as ICAM-1, VCAM-1, and E-selectin, was also suppressed by Ly294002. Altogether, our results confirm the pivotal role of PI3K/Akt pathway in sepsis and its inhibition might be a potential therapeutic target.
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Authors | Peng Wan, Xiang Tan, Yan Xiang, Huasheng Tong, Min Yu |
Journal | Inflammation
(Inflammation)
Vol. 41
Issue 5
Pg. 1815-1824
(Oct 2018)
ISSN: 1573-2576 [Electronic] United States |
PMID | 29956071
(Publication Type: Journal Article)
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Chemical References |
- Chromones
- Morpholines
- CD40 Ligand
- 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- CD40 Ligand
(metabolism)
- Chromones
(pharmacology)
- Endothelial Cells
(pathology)
- Humans
- Metabolic Networks and Pathways
(drug effects, physiology)
- Morpholines
(pharmacology)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Platelet Activation
- Proto-Oncogene Proteins c-akt
(metabolism)
- Sepsis
(metabolism, pathology)
- Signal Transduction
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