[Effects of HCN2 in the development of peripheral neuropathic pain in rats].

Abstract

OBJECTIVE:

To explore the effects of hyperpolarization-activated cyclic nucleotide-gated channels 2(HCN2) in the formation of peripheral neuropathic pain in rats.

METHODS:

Twenty-four healthy adult rats were divided into two groups randomly(n=12):the sham group rats were only isolated the left L4, L5 spinal nerve, the spinal nerve ligation(SNL) group was separated the spinal nerve and performed the corresponding ligation. The behavioral experiments were tested 7 days after operation; The model rats were randomly divided into 3 groups(n=6):① negative group(Saline), intra-plantar injection of saline in left hindpaws; ② positive group(gabapentin, GBPT), intraperitoneal injection of gabapentin; ③ experimental group(ZD7288), intra-plantar injection of ZD7288 in left hindpaws. The behavioral experiments were tested before injection and 1 h, 4 h, 24 h and 48 h after injection; Obtaining the dorsal root ganglion(DRG) of the control group (before operation), sham group and the SNL group(n=6), using qPCR and Western blot to analyze the mRNA and protein of HCN2 in rats' DRG.

RESULTS:

The rat model of neuropathic pain was successfully established. Compared with saline group, GBPT group and ZD7288 group could significantly reduce the symptoms of neuropathic pain in rats after injection 1 h (P<0.01), and there was no difference between GBPT group and ZD7288 group. Compared with control group and sham group, the expression of HCN2 mRNA in SNL group's DRG was significantly increased (P<0.01), and the expression of HCN2 channel protein was also increased significantly (P<0.05).

CONCLUSIONS:

HCN2 is involved in the development of peripheral neuropathic pain and is likely to be a potential new target for the treatment of neuropathic pain.

• Authors: Tao Huang, Bo Fu, Jing Wang, Bin Wang, Shao-Jun Liu, Xie-Chuan Weng
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 33 Issue 4 Pg. 294-298 (Apr 08 2017) ISSN: 1000-6834 [Print] China
• PMID: 29926632 (Publication Type: Journal Article)

Chemical References

• Amines
• Cyclohexanecarboxylic Acids
• Hcn2 protein, rat
• Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
• Potassium Channels
• Pyrimidines
• RNA, Messenger
• ICI D2788
• gamma-Aminobutyric Acid
• Gabapentin

Topics

• Amines (pharmacology)
• Animals
• Cyclohexanecarboxylic Acids (pharmacology)
• Gabapentin
• Ganglia, Spinal
• Hyperalgesia
• Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels (genetics, metabolism)
• Neuralgia (genetics)
• Potassium Channels (genetics, metabolism)
• Pyrimidines (pharmacology)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Spinal Nerves
• gamma-Aminobutyric Acid (pharmacology)