LncRNA LCPAT1 Mediates Smoking/ Particulate Matter 2.5-Induced Cell Autophagy and Epithelial-Mesenchymal Transition in Lung Cancer Cells via RCC2.

Abstract

BACKGROUND/AIMS:

Ecological studies have shown that air pollution and prevalence of cigarette smoking are positively correlated. Evidence also suggests a synergistic effect of cigarette smoking and PM2.5 exposure (Environmental Particulate Matter ≤ 2.5 µm in diameter) on lung cancer risk. We aimed to evaluate the interaction between smoking prevalence and PM2.5 pollution in relation to lung cancer mortality and determine its underlying mechanisms in vitro.

METHODS:

"MOVER" method was used to analyze the interaction between smoking prevalence and PM2.5 pollution in relation to lung cancer mortality. Cell autophagy and malignant behaviors induced by cigarette smoke extract (CSE) and PM2.5 exposure were examined in vitro. Gene expression was examined by qRT-PCR and western blot. RNA and protein interaction was determined using a RNA binding protein immunoprecipitation assay.

RESULTS:

An increased risk for lung cancer death (RERI (the relative excess risk) =0.28) was observed with a synergistic interaction between cigarette smoking and PM2.5 pollution. Cell migration, invasion, EMT (epithelial-mesenchymal transition) and autophagy were elevated when lung cancer cells were treated with CSE and PM2.5 in combination. A lncRNA, named lung cancer progression-association transcript 1 (LCPAT1), was up-regulated after the treatment of CSE and PM2.5, and knocking down the lncRNA impaired the effect of CSE and PM2.5 on lung cancer cells. In addition, LCPAT1 was shown to bind to RCC2, and RCC2 mediated the effect of LCPAT1 on cell autophagy, migration, invasion and EMT in lung cancer.

CONCLUSIONS:

Our results suggest that combined exposure to CSE and PM2.5 induces LCPAT1 expression, which up-regulates autophagy, and promotes lung cancer progression via RCC2.

• Authors: Hongyan Lin, Xiaohong Zhang, Nannan Feng, Ruoyang Wang, Weituo Zhang, Xiaobei Deng, Yu Wang, Xiao Yu, Xiaofei Ye, Lei Li, Ying Qian, Herbert Yu, Biyun Qian
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 47 Issue 3 Pg. 1244-1258 ( 2018) ISSN: 1421-9778 [Electronic] Germany
• PMID: 29913439 (Publication Type: Journal Article)
• Copyright: © 2018 The Author(s). Published by S. Karger AG, Basel.

Chemical References

• Chromosomal Proteins, Non-Histone
• Guanine Nucleotide Exchange Factors
• Neoplasm Proteins
• Particulate Matter
• RCC2 protein, human
• RNA, Long Noncoding
• RNA, Neoplasm

Topics

• Autophagy
• Chromosomal Proteins, Non-Histone (metabolism)
• Epithelial-Mesenchymal Transition
• Female
• Guanine Nucleotide Exchange Factors (metabolism)
• Humans
• Lung Neoplasms (metabolism, pathology)
• Male
• Neoplasm Proteins (metabolism)
• Particulate Matter (toxicity)
• RNA, Long Noncoding (metabolism)
• RNA, Neoplasm (metabolism)
• Smoking (adverse effects)