Esophageal squamous cell carcinoma (ESCC) is highly malignant. Recently, the expression of
myosin 5a, a member of the
myosin superfamily, was reported to be associated with increased invasiveness and
metastasis in many
tumor types. Moreover,
myosin 5a is upregulated by Snail and activated by Akt2, both of which are epithelial-mesenchymal transition (EMT) markers. In this study, we confirmed the expression of
myosin 5a in ESCC surgical specimens and cell lines, revealing its correlation with
tumor invasion, migration, patient prognosis, and expression of EMT-related
proteins. The expression of
myosin 5a,
vimentin, and
E-cadherin was immunohistochemically evaluated in 118 patients with ESCC who underwent
esophagectomy without
chemotherapy or irradiation
therapy prior to surgery. We also investigated ESCC cell migration under
myosin 5a silencing by
siRNA induction. The high expression of
myosin 5a was correlated with
tumor depth,
lymph node metastasis, pathological stage, high
vimentin expression, and low
E-cadherin expression. Patients with high expression of
myosin 5a, including those with pT1
cancer, exhibited significantly worse survival. Moreover, the expression level of
vimentin mRNA and the number of migrated ESCC cells decreased significantly following
myosin 5a silencing. Our findings demonstrate that high expression of
myosin 5a may be an independent prognostic factor in patients with ESCC, even in early invasive
carcinoma, and indicate
myosin 5a has a role in both cell migration and EMT.