Abstract | OBJECTIVE: The production of uric acid in murine white adipose tissue (mWAT), and that such production was augmented in obese mice, was recently reported. However, little is known about the secretion of metabolites associated with purine catabolism in human WAT (hWAT). The present study analyzed this in hWAT. METHODS: Freshly isolated hWAT and mWAT were cultured. The secretion of metabolites associated with purine catabolism was measured. Tissue distribution profiles of genes associated with purine metabolism and metabolite profiling of adipocytes in hypoxia were analyzed. RESULTS: CONCLUSIONS: The present study revealed that hypoxanthine was secreted from human adipose tissue, and the secretion might be increased in local hypoxia.
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Authors | Hirofumi Nagao, Hitoshi Nishizawa, Yoshimitsu Tanaka, Tadafumi Fukata, Tsunekazu Mizushima, Masahiro Furuno, Takeshi Bamba, Yu Tsushima, Yuya Fujishima, Shunbun Kita, Tohru Funahashi, Norikazu Maeda, Masaki Mori, Eiichiro Fukusaki, Iichiro Shimomura |
Journal | Obesity (Silver Spring, Md.)
(Obesity (Silver Spring))
Vol. 26
Issue 7
Pg. 1168-1178
(07 2018)
ISSN: 1930-739X [Electronic] United States |
PMID | 29877616
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2018 The Obesity Society. |
Chemical References |
- Xanthine
- Uric Acid
- Hypoxanthine
- Xanthine Dehydrogenase
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Topics |
- 3T3-L1 Cells
- Adipocytes
(metabolism)
- Adipose Tissue
(metabolism)
- Adipose Tissue, White
(metabolism)
- Adolescent
- Adult
- Animals
- Cells, Cultured
- Humans
- Hypoxanthine
(metabolism)
- Hypoxia
(genetics, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Uric Acid
(metabolism)
- Xanthine
(metabolism)
- Xanthine Dehydrogenase
(genetics, metabolism)
- Young Adult
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