The action mechanism of long non-coding
ribonucleic acid-homeobox transcript antisense
ribonucleic acid (
lncRNA-HOTAIR) in the regulation of the Wnt signaling pathway on the drug resistance of
non-small cell lung cancer was investigated. Forty eight patients with
non-small cell lung cancer, who were treated with
cisplatin (DDP) as
neoadjuvant chemotherapy, were selected from the specimen bank of the Department of Pathology of Peking Union Medical College Hospital. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the
messenger RNA (
mRNA) level of
lncRNA-HOTAIR in
cancer and
cancer-adjacent tissues. The correlation curve of the expression of
lncRNA-HOTAIR with the overall survival (OS) was plotted using the Kaplan-Meier method. NCI-H1299 DDP-resistant cell lines were constructed, and the half maximal inhibitory concentration (IC50) value was measured. The expression of lnc-HOTAIR in NCI-H1299/DDP cells was detected by the target interference of
small interfering RNA (
siRNA). The effect of si-HOTAIR on cell resistance was detected by Cell Counting Kit-8 (CCK-8). Western blot analysis was used to detect the effects of si-HOTAIR on
multidrug resistance proteins,
multidrug resistance-associated protein 1 (
MRP1) and multidrug resistance 1 (MDR1), and Wnt signaling pathways, Wnt3a,
adenomatous polyposis coli (APC) and β-
catenin. The
mRNA level of
lncRNA-HOTAIR in
cancer tissues was significantly higher than that in
cancer-adjacent tissues (P<0.05), and the high expression of
lncRNA-HOTAIR indicated that the OS of patients was shortened (P<0.05). The IC50 of NCI-H1299/DDP cells inhibiting DDP was 127.82 µM, which was significantly higher than that of parental NCI-H1299 cells (IC50=8.40 µM) (P<0.05). si-HOTAIR interference significantly decreased the sensitivity of cells to DDP, the IC50 of cells was decreased from 131.85 to 44.34 µM (P<0.05), the expression levels of
MRP1 and MDR1 were significantly decreased, and the activation of Wnt signaling pathway was significantly inhibited (P<0.05). Thus,
lncRNA-HOTAIR plays an important role in the occurrence and development of
non-small cell lung cancer, and it may be an important factor in the clinical prognosis of patients with
non-small cell lung cancer.