Abstract | BACKGROUND/AIMS: METHODS: Ultra-performance liquid chromatography/mass spectrometry was used to investigate the fecal and serum metabolic profiles of 20 healthy controls and 31 HD patients before and after taking medicinal charcoal tablets for 3 months. RESULTS: There were distinct metabolic variations between the HD patients and healthy controls both in the feces and serum according to multivariate data analysis. Metabolic disturbances of alanine, aspartate and glutamate metabolism, arginine and proline metabolism figured prominently in the serum. However, in the feces, alterations of tryptophan metabolism, lysine degradation and beta-alanine metabolism were pronounced, and the levels of several amino acids ( leucine, phenylalanine, lysine, histidine, methionine, tyrosine, and tryptophan) were increased dramatically. Nineteen fecal metabolites and 21 serum metabolites were also identified as biomarkers that contributed to the metabolic differences. Additionally, medicinal charcoal treatment generally enabled the serum and fecal metabolomes of the HD patients to draw close to those of the control subjects, especially the serum metabolic profile. CONCLUSION: Parallel fecal and serum metabolomics uncovered the systematic metabolic variations of HD patients, especially disturbances in amino acid metabolism in the colon. Medicinal charcoal tablets had an impact on the serum and fecal metabolomes of HD patients, but their exact effects still need to be studied further.
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Authors | Sixiu Liu, Shanshan Liang, Hua Liu, Lei Chen, Lingshuang Sun, Meng Wei, Hongli Jiang, Jing Wang |
Journal | Kidney & blood pressure research
(Kidney Blood Press Res)
Vol. 43
Issue 3
Pg. 755-767
( 2018)
ISSN: 1423-0143 [Electronic] Switzerland |
PMID | 29804117
(Publication Type: Journal Article)
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Copyright | © 2018 The Author(s). Published by S. Karger AG, Basel. |
Chemical References |
- Amino Acids
- Tablets
- Charcoal
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Topics |
- Adult
- Amino Acids
(metabolism)
- Case-Control Studies
- Charcoal
(therapeutic use)
- Chromatography, High Pressure Liquid
- Colon
(metabolism)
- Feces
(chemistry)
- Female
- Humans
- Male
- Mass Spectrometry
- Metabolome
(drug effects)
- Metabolomics
(methods)
- Middle Aged
- Renal Dialysis
- Renal Insufficiency, Chronic
(metabolism)
- Serum
(drug effects, metabolism)
- Tablets
(chemistry)
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