Objective:To investigate the mechanism between epithelial-mesenchymal transition (EMT) and
cisplatin induced resistant cell subline and the malignant
biological characteristics, to explore EMT in human hep-2 laryngeal resistant cells. Method:Using
cisplatin-resistant cells (hep-2/CDDP) and non-resistant cells (hep-2) established in our previous study; the invasion and migration
biological behaviors were detected by transwell and scratch assay; the expressions of
E-cadherin, Zo-1, Snail, Slug, Twist1, Vimentinon in the
mRNA level were detected by RT-qPCR and the
protein level by Western blot. Result:Transwell and scratch assay show the invasion and migration behaviors were increased in hep-2/CDDP cells (P<0.05), the epithelial marker
E-cadherin and Zo-1 were downregulated in hep-2/CDDP cells (all P<0.05),
transcription factor Snail, Slug were upregulated in
mRNA and
protein level (all P<0.01) while Twist1 had no significant changed in
protein level (P>0.05), the expression of mesenchymal marker
Vimentin was also increased in
mRNA and
protein levels in
cisplatin resistant cells (P<0.01). It was confirmed that the hep-2/CDDP cells possessed EMT phenotypes. Conclusion:The
cisplatin resistant
laryngeal cancer cells perform higherinvasion and migration
biological behaviors,and the mechanisms of increased ability of invasion and migration induced by
cisplatin was associated to eEMT, study on signal path related to EMT may overcome
cisplatin resistance and reduce invasion and migration behaviors.