5-Methoxytryptophan (5-MTP) is a
tryptophan metabolite with recently discovered anti-inflammatory and
tumor-suppressing activities. Its synthesis is catalyzed by a
hydroxyindole O-methyltransferase (
HIOMT)-like
enzyme. However, the exact identity of this
HIOMT in human cells remains unclear. Human
HIOMT exists in several alternatively spliced
isoforms, and we hypothesized that 5-MTP-producing
HIOMT is a distinct
isoform. Here, we show that human fibroblasts and
cancer cells express the HIOMT298
isoform as contrasted with the expression of the HIOMT345
isoform in pineal cells. Sequencing analysis of the cloned
isoforms revealed that HIOMT298 is identical to the sequence of a previously reported truncated
HIOMT isoform. Of note, HIOMT298 expression was reduced in
cancer cells and tissues. Stable transfection of A549
cancer cells with HIOMT298 restored
HIOMT expression to normal levels, accompanied by 5-MTP production. Furthermore, HIOMT298 transfection caused a
tryptophan-metabolic switch from
serotonin to 5-MTP production. To determine the in vivo relevance of this alteration, we compared growth and lung
metastasis of HIOMT298-transfected A549 cells with those of vector- or untransfected A549 cells as controls in a murine xenograft model. Of note, the HIOMT298-transfected A549 cells exhibited slower growth and lower
metastasis than the controls. Our findings provide insight into the crucial role of HIOMT298 in 5-MTP production in cells and in inhibiting
cancer progression and highlight the potential therapeutic value of 5-MTP for managing
cancer.