Immune response and protective effect against spring viremia of carp virus induced by intramuscular vaccination with a SWCNTs-DNA vaccine encoding matrix protein.
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| Abstract |
To elicit the immune protective of vaccine against the highly contagious and pathogenic disease caused by spring viremia of carp virus (SVCV), a novel functionalized single-walled carbon nanotubes (SWCNTs) were applied as a delivery vehicle for DNA vaccine. In this study, we report a SWCNTs-DNA vaccine encoding matrix protein of SVCV which, when injected in the muscle at a dose of 10 μg SWCNTs-pcDNA-M vaccine, confers up to 51.3% protection against intraperitoneal challenge with SVCV. In addition, SWCNTs as a promising vehicle can enhance about 17.5% of the immune protective effect in SWCNTs-pcDNA-M vaccinated common carp compared with fish injected with naked pcDNA-M DNA vaccine. In addition, serum antibody production, none specific immunity parameters (complement activity, superoxide dismutase activity (SOD), acid phosphatase activity (ACP) and alkaline phosphatase activity (AKP)) and immune-related genes were used to verify the enhancement immune response induced in SWCNTs-pcDNA-M vaccinated fish, herein all these mentioned immune activities were significantly enhanced after immunization. Thereby, it is revealed that the M gene of SVCV could be used as an antigen for DNA vaccine constructs, and SWCNTs could be a candidate DNA vaccine carrier to enhance the immunological response against fish disease.
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| Authors | Chen Zhang, Zhao Zhao, Gao-Yang Liu, Jian Li, Gao-Xue Wang, Bin Zhu |
| Journal | Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 79 Pg. 256-264 (Aug 2018) ISSN: 1095-9947 [Electronic] England |
| PMID | 29777766 (Publication Type: Journal Article) |
| Copyright | Copyright © 2018 Elsevier Ltd. All rights reserved. |
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