Abstract | OBJECTIVE:
Osteosarcoma is the most frequent primary bone malignancy that affects young adults and adolescents around the world. Increasing evidence suggests that dysfunctions of microRNAs ( miRNAs) used to play an important role in human cancers. We aimed at evaluating the potential function of miR-16 and verify its influence on the function of RAB23 in osteosarcoma. PATIENTS AND METHODS: miR-16 expressions in osteosarcoma tissues and cell lines were examined using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Transwell chambers were conducted to detect the miR-16 effects on osteosarcoma cells migration and invasion. Meanwhile, Western blot and luciferase assays were performed to validate RAB23 as miR-16 targets. RESULTS: miR-16 was down-regulated in osteosarcoma cell lines (MG63, SAOS-2, U2OS, and SOSP-9607) and osteosarcoma specimens, while RAB23 expression was higher in tumor tissues. Ectopic over-expression of miR-216 in osteosarcoma cells could inhibit cells migration and invasion. RAB23 was confirmed as a direct target of miR-16 and the inverse relationship between them was also observed. Over-expression of RAB23 ablates the inhibitory effects of miR-16. CONCLUSIONS: miR-16 inhibited cancer migration and invasion, and promoted the RAB23 expression in osteosarcoma. This newly identified miR-16/RAB23 axis may provide new insight into the pathogenesis and represents a potential therapeutic target for osteosarcoma.
|
Authors | Z-H Jiao, J-D Wang, X-J Wang |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 22
Issue 9
Pg. 2598-2605
(05 2018)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 29771408
(Publication Type: Journal Article)
|
Chemical References |
- 3' Untranslated Regions
- MIRN16 microRNA, human
- MicroRNAs
- RAB23 protein, human
- rab GTP-Binding Proteins
|
Topics |
- 3' Untranslated Regions
- Binding Sites
- Bone Neoplasms
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
- Epithelial-Mesenchymal Transition
- Gene Expression Regulation, Neoplastic
- Humans
- MicroRNAs
(genetics, metabolism)
- Neoplasm Invasiveness
- Osteosarcoma
(genetics, metabolism, pathology)
- Signal Transduction
- rab GTP-Binding Proteins
(genetics, metabolism)
|