MicroRNA-873 Promotes Cell Proliferation, Migration, and Invasion by Directly Targeting TSLC1 in Hepatocellular Carcinoma.

Abstract

BACKGROUND/AIMS:

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and has the third highest mortality rate among all cancers. MicroRNAs are a class of endogenous, single-stranded short noncoding RNAs. The purpose of this study was to study the role of microRNA-873 in HCC.

METHODS:

The expression of miRNA-873 and tumor suppressor in lung cancer 1 (TSLC1) in HCC tissues and cell lines was detected by real-time quantitative RT-PCR (RT-qPCR) or western blot. A CCK-8 assay was used to examine cell proliferation; flow cytometry was used to assess the cell cycle; the Transwell migration assay was used to test for metastasis. Luciferase assays were performed to assess whether TSLC1 was a novel target of miRNA-873.

RESULTS:

We showed that miRNA-873 was upregulated in HCC tissues and cell lines compared with the normal control. Knockdown of miRNA-873 inhibited the growth and metastasis of HepG2 and accelerated G1 phase arrest, while overexpression of miRNA-873 had the opposite effect. The dual-luciferase reporter assays revealed that TSLC1 was a novel target of miRNA-873. Further study showed that TSLC1 was decreased in HCC tissues and cell lines. There was a negative correlation between the expression levels of TSLC1 and miRNA-873. The effect of miRNA-873 overexpression was neutralized by TSLC1. We also found that miRNA-873 activated the PI3K/AKT/mTOR signaling pathway and promoted HCC.

CONCLUSIONS:

Our data demonstrated that miRNA-873 promoted HCC progression by targeting TSLC1 and provided a new target for the therapy of HCC.

• Authors: Guoyong Han, Long Zhang, Xuhao Ni, Zhiqiang Chen, Xiongxiong Pan, Qin Zhu, Shu Li, Jindao Wu, Xinli Huang, Xuehao Wang
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 46 Issue 6 Pg. 2261-2270 ( 2018) ISSN: 1421-9778 [Electronic] Germany
• PMID: 29734164 (Publication Type: Journal Article)
• Copyright: © 2018 The Author(s). Published by S. Karger AG, Basel.

Chemical References

• CADM1 protein, human
• Cell Adhesion Molecule-1
• MIRN873 microRNA, human
• MicroRNAs

Topics

• Carcinoma, Hepatocellular (genetics, pathology)
• Cell Adhesion Molecule-1 (genetics)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Liver Neoplasms (genetics, pathology)
• Male
• MicroRNAs (genetics)
• Middle Aged
• Neoplasm Invasiveness (genetics, pathology)