Physicochemical properties of polysaccharides from Lentinus edodes under high pressure cooking treatment and its enhanced anticancer effects.

Abstract

This study was to investigate the physicochemical properties and anticancer effects of polysaccharides from Lentinus edodes extracted under high pressure cooking treatment (HPLPS) in vitro and in vivo. The extraction efficiency was improved. The main molecular weight of HPLPS was about 540 and about 227 kDa. And the inhibitory effects on HepG2 and HeLa cells of HPLPS were significantly increased (p < 0.05). The in vivo anticancer effect on H22 tumor bearing mice model was evaluated. The tumor growth inhibitory rate of HPLPS-H was 67.66%. The activities of ALT and AST were decreased. The activities of SOD, CAT, GSH-Px were notably increased. The expressions of IL-2 and TNF-α were increased while the expression of VEGF was decreased. These results suggested that high pressure-assisted extracted polysaccharides from Lentinus edodes might be effectively used for the treatment of hepatocellular carcinoma through its antioxidant and immunomodulatory effects.

• Authors: Weiwei Li, Jingya Wang, Zhongqin Chen, Xudong Gao, Yue Chen, Zihan Xue, Qingwen Guo, Qiqi Ma, Haixia Chen
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 115 Pg. 994-1001 (Aug 2018) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 29694839 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Cytokines
• Fungal Polysaccharides

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Cell Proliferation (drug effects)
• Chemical Phenomena
• Cooking (methods)
• Cytokines (metabolism)
• Fungal Polysaccharides (chemistry, pharmacology)
• HeLa Cells
• Humans
• Mice
• Molecular Weight
• Pressure
• Shiitake Mushrooms (chemistry)