Allium Sativum Methanolic Extract (garlic) Improves Therapeutic Efficacy of Albendazole Against Hydatid Cyst: In Vivo Study.

Abstract

After gas chromatography and mass spectrometry of prepared methanolic extract of Allium sativum, 40 laboratory BALB/c mice were infected intraperitoneally by injection of 1,500 viable protoscoleces. Five months after infection, the infected mice were allocated into four treatment groups, including 1- Albendazole (100 mg/kg); 2- Allium sativum methanolic extract (10 mL/L); 3- A. sativum methanolic extract (10 mL/L) + Albendazole (50 mg /kg); and 4- untreated control group. After 30 days of daily treatment, total number and weight of cysts and size of the largest cyst as well as blood serum bilirubin and liver enzymes were compared between the mice of different groups.The total number and weight of cysts and size of the largest cyst were significantly lower in treated groups A. sativum 10 mL/L + Albendazole 50 and Albendazole 100 in comparison to those of the control group (p < 0.05).The activity of alanine aminotransferase (ALT) enzyme and bilirubin concentration were significantly lower in the mice treated with A. sativum 10 mL/L and A. sativum 10 mL/L + Albendazole 50, when compared to the control group. In addition, bilirubin concentration revealed significant decrease in A. sativum 10 mL/L and A. sativum 10 mL/L + Albendazole 50 groups, when compared to the Albendazole group. In conclusion, administration of A. sativum 10 mL/L improved the anti-hydatidosis activity of Albendazole 50 mg /kg, due to parasitological effects similar to Albendazole 100 mg /kg but less hepatotoxic effects.

• Authors: K H Haji Mohammadi, M Heidarpour, H Borji
• Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (J Invest Surg) Vol. 32 Issue 8 Pg. 723-730 (Dec 2019) ISSN: 1521-0553 [Electronic] United States
• PMID: 29693456 (Publication Type: Journal Article)

Chemical References

• Anticestodal Agents
• Plant Extracts
• Albendazole
• Methanol

Topics

• Administration, Oral
• Albendazole (pharmacology, therapeutic use)
• Animals
• Anticestodal Agents (pharmacology, therapeutic use)
• Disease Models, Animal
• Drug Synergism
• Drug Therapy, Combination (methods)
• Echinococcosis (drug therapy, parasitology)
• Echinococcus granulosus (drug effects, pathogenicity)
• Garlic (chemistry)
• Humans
• Methanol (chemistry)
• Mice
• Mice, Inbred BALB C
• Plant Extracts (pharmacology, therapeutic use)
• Treatment Outcome