Anti-Cancer Effects of Paris Polyphylla Ethanol Extract by Inducing Cancer Cell Apoptosis and Cycle Arrest in Prostate Cancer Cells.

Abstract

OBJECTIVE:

To evaluate the potential anti-prostate cancer effects of Paris polyphylla ethanol extract (PPEE) and its underlying mechanisms.

MATERIALS AND METHODS:

The anti-proliferation activity of PPEE was tested on PC3 and DU145 cells using Cell Counting Kit-8 assay. The pro-apoptotic and cell cycle arrest effects of PPEE were confirmed by flow cytometry. Apoptosis of prostate cancer cells was induced by PPEE through endogenous and exogenous pathways. A mouse xenograft model was used to examine its anti-prostate cancer effects in vivo.

RESULTS:

We found that the IC50 of PPEE on PC3 cells was 3.98 µg/ml and the IC50 of PPEE on DU145 cells was 8 µg/ml. PPEE induced prostate cancer cell apoptosis in a concentration dependent manner, through endogenous and exogenous pathways. PPEE induced PC3 cell cycle arrest in G0/G1 and G2/M phases, while in DU145cell it induced cell arrest in the G0/G1 phase. PPEE inhibited the growth of prostate cancer cells in vivo.

CONCLUSION:

PPEE could inhibit prostate cancer growth in vitro and in vivo, induce apoptosis of prostate cancer cells, and cause cell cycle arrest, which laid the foundation for further research on the anti-tumor mechanism of PPEE.

• Authors: Denglu Zhang, Kailin Li, Chao Sun, Guangshang Cao, Yuanfu Qi, Zhaomin Lin, Yanxia Guo, Zhiyong Liu, Yuan Chen, Jiaxin Liu, Guanghui Cheng, Peng Wang, Lu Zhang, Jianye Zhang, Jiliang Wen, Dawei Xu, Feng Kong, Shengtian Zhao
• Journal: Current urology (Curr Urol) Vol. 11 Issue 3 Pg. 144-150 (Mar 2018) ISSN: 1661-7649 [Print] United States
• PMID: 29692694 (Publication Type: Journal Article)