[Immunologic mechanisms of Yinhua Pinggan granule and San-ao decoction against influenza virus in vivo].

Abstract

This paper aimed to investigate the effect of Yinhua Pinggan granule and San-ao decoction on the immunologic mechanisms of influenza viral pneumonia mice in vivo, in order to study the activity of the combined administration of different formulas on influenza A/H1N1 virus. The model of pneumonia was established in mice through nasal dropping influenza virus, and then divided randomly into five groups: normal control group, influenza virus model group, oseltamivir control group, Yinhua Pinggan granule group, and San-ao decoction group. The animals were put to death at the 5th day after gavage administration with the corresponding drugs. The contents in mice serum of TNF-α, IL-6 and IFN-γ were respectively measured by ELISA. The mRNA expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in lung tissues were respectively detected by RT-PCR. The protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues were determined by immunohistochemical analysis, respectively. According to the results, Yinhua Pinggan granule and San-ao decoction could significantly decrease the levels of TNF-α and IL-6, increase the level of IFN-γ in mice serum of lung tissues, significantly reduce the gene expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in influenza virus-infected mice lung tissues, and significantly reduce the protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues. Furthermore, the regulatory effect of Yinhua Pinggan granule was superior to that of San-ao decoction. In conclusion, Yinhua Pingan granule and San-ao decoction have the therapeutic effect on pneumonia mice infected by H1N1 virus in vivo. The anti-influenza mechanisms of Yinhua Pinggan granule and San-ao decoction may be the results of interactions by regulating the immunologic function of influenza virus-infected mice and TLR3/7 signaling pathway with multiple links of the gene and protein expressions. Moreover, the combined administration of warm-natured and cold-natured Yinhua Pinggan granule with the effects of detoxification and exhalation has a better effect than the single administration of warm-natured San-ao decoction.

• Authors: Hai-Xia Du, Hui-Fen Zhou, Yu He, Jie-Hong Yang, Yi-Yu Lu, Yi-Chen Fan, Hai-Tong Wan
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 43 Issue 5 Pg. 1028-1033 (Mar 2018) ISSN: 1001-5302 [Print] China
• PMID: 29676104 (Publication Type: Journal Article)
• Copyright: Copyright© by the Chinese Pharmaceutical Association.

Chemical References

• Drugs, Chinese Herbal
• Membrane Glycoproteins
• Myd88 protein, mouse
• Myeloid Differentiation Factor 88
• San-Ao decoction
• TLR3 protein, mouse
• Tlr7 protein, mouse
• Toll-Like Receptor 3
• Toll-Like Receptor 7
• yinhuapinggan

Topics

• Animals
• Drugs, Chinese Herbal (pharmacology)
• Influenza A Virus, H1N1 Subtype
• MAP Kinase Signaling System
• Membrane Glycoproteins (metabolism)
• Mice
• Myeloid Differentiation Factor 88 (metabolism)
• Orthomyxoviridae Infections (drug therapy)
• Pneumonia, Viral (drug therapy)
• Toll-Like Receptor 3 (metabolism)
• Toll-Like Receptor 7 (metabolism)