Fabrication of Sericin/Agrose Gel Loaded Lysozyme and Its Potential in Wound Dressing Application.

Abstract

Sericin is a biomaterial resource for its significant biodegradability, biocompatibility, hydrophilicity, and reactivity. Designing a material with superabsorbent, antiseptic, and non-cytotoxic wound dressing properties is advantageous to reduce wound infection and promote wound healing. Herein, we propose an environment-friendly strategy to obtain an interpenetrating polymer network gel through blending sericin and agarose and freeze-drying. The physicochemical characterizations of the sericin/agarose gel including morphology, porosity, swelling behavior, crystallinity, secondary structure, and thermal property were well characterized. Subsequently, the lysozyme loaded sericin/agarose composite gel was successfully prepared by the solution impregnation method. To evaluate the potential of the lysozyme loaded sericin/agarose gel in wound dressing application, we analyzed the lysozyme loading and release, antimicrobial activity, and cytocompatibility of the resulting gel. The results showed the lysozyme loaded composite gel had high porosity, excellent water absorption property, and good antimicrobial activities against Escherichia coli and Staphylococcus aureus. Also, the lysozyme loaded gel showed excellent cytocompatibility on NIH3T3 and HEK293 cells. So, the lysozyme loaded sericin/agarose gel is a potential alternative biomaterial for wound dressing.

• Authors: Meirong Yang, Yejing Wang, Gang Tao, Rui Cai, Peng Wang, Liying Liu, Lisha Ai, Hua Zuo, Ping Zhao, Ahmad Umar, Chuanbin Mao, Huawei He
• Journal: Nanomaterials (Basel, Switzerland) (Nanomaterials (Basel)) Vol. 8 Issue 4 (Apr 13 2018) ISSN: 2079-4991 [Print] Switzerland
• PMID: 29652825 (Publication Type: Journal Article)