Clonorchis sinensis omega-class glutathione transferases are reliable biomarkers for serodiagnosis of clonorchiasis and opisthorchiasis.

Abstract

OBJECTIVES:

To determine the potential for immunodiagnostic application of two recombinant forms of Clonorchis sinensis omega-class glutathione transferases (rCsGSTo1 and rCsGSTo2) against human small liver-fluke C. sinensis and Opisthorchis viverrini infections.

METHODS:

Specific antibody levels against rCsGSTo1 and rCsGSTo2 in patients' sera of egg-positive opisthorchiasis (n = 87) and clonorchiasis (n = 120), as well as those in sera from patients with other helminthic infections (n = 252) and healthy controls (n = 40) were retrospectively analysed by ELISA.

RESULTS:

We observed highly positive correlation coefficients between specific antibody levels against rCsGSTo1 and rCsGSTo2 and egg counts per gramme of faeces (EPG) of patients with opisthorchiasis (n = 87; r = 0.88 for rCsGSTo1 and r = 0.90 for rCsGSTo2). Sera from opisthorchiasis patients whose EPG counts >100 (n = 43) revealed high antibody titres against both antigens. Patients' sera with low EPG counts (<100, n = 44) also exhibited reliable sensitivities of 93.2% and 97.7% for rCsGSTo1 and rCsGSTo2, respectively. Sera from clonorchiasis patients showed sensitivities of 90% (108/120 samples) and 89.2% (107/120 sera) for rCsGSTo1 and rCsGSTo2. Overall diagnostic sensitivities for liver-fluke infections were 92.3% for rCsGSTo1 (191/207 samples) and 93.2% for rCsGSTo2 (193/207 samples). Specificities were 89.7% (rCsGSTo1) and 97.6% (rCsGSTo2).

CONCLUSIONS:

Detection of specific antibody levels against rCsGSTo1 or rCsGSTo2 might be promising for the serodiagnosis of patients infected with these two phylogenetically close carcinogenic liver-flukes.

• Authors: J-G Kim, C-S Ahn, B Sripa, K S Eom, I Kang, W-M Sohn, Y Nawa, Y Kong
• Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases (Clin Microbiol Infect) Vol. 25 Issue 1 Pg. 109.e1-109.e6 (Jan 2019) ISSN: 1469-0691 [Electronic] England
• PMID: 29649604 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Helminth
• Biomarkers
• Glutathione Transferase

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Animals
• Antibodies, Helminth (blood)
• Biomarkers (blood)
• Child
• Clonorchiasis (blood, diagnosis)
• Clonorchis sinensis (enzymology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Feces (parasitology)
• Female
• Glutathione Transferase (blood)
• Humans
• Male
• Middle Aged
• Opisthorchiasis (blood, diagnosis)
• Parasite Egg Count
• Retrospective Studies
• Sensitivity and Specificity
• Serologic Tests
• Young Adult