Abstract |
Circular RNAs ( circRNAs) are highly expressed in eukaryotic cells and regulate physiological and pathophysiological processes. However, the role of circRNAs in cerebral ischemia-reperfusion (I/R) injury remains largely unknown. In this study, we found that circ_008018 level was higher in the cortical tissue of mice with middle cerebral artery occlusion as compared to those in the sham group 24 h after reperfusion. Knockdown of circ_008018 attenuated cerebral I/R-induced brain tissue damage and neurological deficits in mice by inducing microRNA miR-99a overexpression. The decreased phosphorylation of Akt and glycogen synthase kinase 3β caused by I/R was partly reversed by circ_008018 silencing or miR-99a overexpression. Taken together, these results provide new insight into the mechanisms of apoptosis resulting from cerebral I/R injury and suggest that targeted inhibition of circ_008018 can protect against subsequent neurological damage.
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Authors | Xuelian Yang, Haifeng Ji, Yulan Yao, Xiaoyin Lai, Yufeng Jiang, Dayu Wu, Liying Cai, Wei Zhu, Xiaju Gu, Rongguo Hu, Longxuan Li, Lijuan Xu, Mei Jiang |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 499
Issue 4
Pg. 758-764
(05 23 2018)
ISSN: 1090-2104 [Electronic] United States |
PMID | 29605297
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2018 Elsevier Inc. All rights reserved. |
Chemical References |
- MicroRNAs
- Mirn99 microRNA, mouse
- RNA, Circular
- RNA
- Glycogen Synthase Kinase 3 beta
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Base Sequence
- Brain Ischemia
(genetics, pathology, prevention & control)
- Down-Regulation
(genetics)
- Gene Silencing
- Glycogen Synthase Kinase 3 beta
(genetics)
- Male
- Mice, Inbred C57BL
- MicroRNAs
(genetics, metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- RNA
(genetics, metabolism)
- RNA, Circular
- Reperfusion Injury
(genetics, pathology, prevention & control)
- Signal Transduction
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