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Downregulation of circ_008018 protects against cerebral ischemia-reperfusion injury by targeting miR-99a.

Abstract
Circular RNAs (circRNAs) are highly expressed in eukaryotic cells and regulate physiological and pathophysiological processes. However, the role of circRNAs in cerebral ischemia-reperfusion (I/R) injury remains largely unknown. In this study, we found that circ_008018 level was higher in the cortical tissue of mice with middle cerebral artery occlusion as compared to those in the sham group 24 h after reperfusion. Knockdown of circ_008018 attenuated cerebral I/R-induced brain tissue damage and neurological deficits in mice by inducing microRNA miR-99a overexpression. The decreased phosphorylation of Akt and glycogen synthase kinase 3β caused by I/R was partly reversed by circ_008018 silencing or miR-99a overexpression. Taken together, these results provide new insight into the mechanisms of apoptosis resulting from cerebral I/R injury and suggest that targeted inhibition of circ_008018 can protect against subsequent neurological damage.
AuthorsXuelian Yang, Haifeng Ji, Yulan Yao, Xiaoyin Lai, Yufeng Jiang, Dayu Wu, Liying Cai, Wei Zhu, Xiaju Gu, Rongguo Hu, Longxuan Li, Lijuan Xu, Mei Jiang
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 499 Issue 4 Pg. 758-764 (05 23 2018) ISSN: 1090-2104 [Electronic] United States
PMID29605297 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2018 Elsevier Inc. All rights reserved.
Chemical References
  • MicroRNAs
  • Mirn99 microRNA, mouse
  • RNA, Circular
  • RNA
  • Glycogen Synthase Kinase 3 beta
  • Proto-Oncogene Proteins c-akt
Topics
  • Animals
  • Base Sequence
  • Brain Ischemia (genetics, pathology, prevention & control)
  • Down-Regulation (genetics)
  • Gene Silencing
  • Glycogen Synthase Kinase 3 beta (genetics)
  • Male
  • Mice, Inbred C57BL
  • MicroRNAs (genetics, metabolism)
  • Phosphatidylinositol 3-Kinases (metabolism)
  • Proto-Oncogene Proteins c-akt (metabolism)
  • RNA (genetics, metabolism)
  • RNA, Circular
  • Reperfusion Injury (genetics, pathology, prevention & control)
  • Signal Transduction

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