Gene silencing of heparanase results in suppression of invasion and migration of gallbladder carcinoma cells.

Abstract

This study investigated the effect of transcriptional gene silencing of the heparanase gene on standard gallbladder carcinoma cells (GBC-SD). The miRNAs targeting the promoter region and coding region of the heparanase gene were designed and synthesized. We transfected four recombinant miRNA vectors into GBC-SD. We performed the wound healing assays and invasion assays. The result shows that the heparanase expression was significantly decreased by recombinant vectors in transfected GBC-SD cells (p < 0.01), of which pmiR-Hpa-2 showed best interference effect (p < 0.05). The penetrated and migrating cells numbers and adherence rate of GBC-SD cells were significantly decreased by pmiR-Hpa-2 (p < 0.05).

• Authors: Hao Jin, Min Cui
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 82 Issue 7 Pg. 1116-1122 (Jul 2018) ISSN: 1347-6947 [Electronic] England
• PMID: 29598788 (Publication Type: Journal Article)

Chemical References

• MicroRNAs
• DNA
• heparanase
• Glucuronidase

Topics

• Cell Adhesion
• Cell Line, Tumor
• DNA (genetics)
• Gallbladder Neoplasms (genetics, pathology)
• Gene Silencing
• Genetic Vectors
• Glucuronidase (genetics)
• Humans
• MicroRNAs (genetics)
• Neoplasm Invasiveness (genetics)
• Neoplasm Metastasis (genetics)
• Plasmids
• Promoter Regions, Genetic
• Transfection
• Wound Healing (genetics)