Curcumin and Rutin Down-regulate Cyclooxygenase-2 and Reduce Tumor-associated Inflammation in HPV16-Transgenic Mice.

Abstract

AIM:

Cyclo-oxygenase-2 (COX2) plays a prominent role in carcinogenesis. This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice.

MATERIALS AND METHODS:

Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16-/- no treatment, n=12; HPV16+/- no treatment, n=13; HPV16+/- rutin, n=12; HPV16+/- curcumin, n=13. HPV16-induced skin lesions and their inflammatory infiltrates were studied histologically. COX2 expression was assessed immunohistochemically.

RESULTS:

Rutin reduced COX2 expression in the dermis (immunostaining score 7.83 versus 11.25 in untreated HPV16-transgenic mice) and epidermis (4.5 versus 10.0). Curcumin led to dermal and epidermal scores of 10.5 and 4.5. Both compounds reduced leukocytic infiltration, but neither prevented epidermal dysplasia.

CONCLUSION:

COX2 expression in HPV16-induced lesions may be modulated by nutraceuticals, reducing tumor-associated inflammation. However, this was not sufficient to block carcinogenesis, calling for additional studies focused on combination therapies.

• Authors: Magda S S Moutinho, Sofia Aragão, Diogo Carmo, Fátima Casaca, Sandra Silva, Joana Ribeiro, Hugo Sousa, Isabel Pires, Felisbina Queiroga, Bruno Colaço, Rui Medeiros, Paula A Oliveira, Carlos Lopes, Margarida M S M Bastos, Rui M Gil DA Costa
• Journal: Anticancer research (Anticancer Res) Vol. 38 Issue 3 Pg. 1461-1466 (03 2018) ISSN: 1791-7530 [Electronic] Greece
• PMID: 29491072 (Publication Type: Journal Article)
• Copyright: Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Rutin
• Cyclooxygenase 2
• Curcumin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Curcumin (pharmacology)
• Cyclooxygenase 2 (metabolism)
• Disease Progression
• Down-Regulation (drug effects)
• Female
• Human papillomavirus 16 (genetics, physiology)
• Humans
• Inflammation (complications, metabolism, prevention & control)
• Leukocytes (drug effects, pathology)
• Mice, Transgenic
• Papillomavirus Infections (complications, virology)
• Rutin (pharmacology)
• Skin (drug effects, metabolism, pathology)
• Skin Neoplasms (complications, metabolism, prevention & control)