Fluorescein Labeled Leukocytes for in vivo Imaging of Retinal Vascular Inflammation and Infiltrating Leukocytes in Laser-Induced Choroidal Neovascularization Model.

Abstract

Purpose: To study the effect of anti-VEGF treatment on retinal inflammation in a laser-induced CNV rodent model.Methods: Leukocytes labeled with 1% sodium fluorescein were injected into the laser-induced CNV (wild type C57BL/6) mice at days 4 (baseline), 7, 14, and 19. At baseline intravitreally 3 mice received 1× PBS, and 3 mice received anti-VEGF. FFA, OCT, and SLO were performed at each time point to assess the CNV pathophysiology and inflammatory response.Results: Fluorescein leakage, SRF, and leukocyte infiltration were observed at baseline in both the groups before injection. From days 7 to 19, leukocyte infiltration and SRF were noted in the 1× PBS group, but limited or no SRF and leukocyte infiltration was observed in the anti-VEGF group.Conclusions: Leukocyte infiltration was established as an in vivo imaging inflammatory marker and along with FFA and OCT showed response to anti-VEGF therapy in laser-induced CNV model.

• Authors: Rupesh Agrawal, Sai Bo Bo Tun, Praveen Kumar Balne, Hong-Yuan Zhu, Neha Khandelwal, Veluchamy A Barathi
• Journal: Ocular immunology and inflammation (Ocul Immunol Inflamm) Vol. 28 Issue 1 Pg. 7-13 ( 2020) ISSN: 1744-5078 [Electronic] England
• PMID: 29470933 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Fluorescein

Topics

• Animals
• Choroid (blood supply)
• Choroidal Neovascularization (diagnosis, etiology)
• Disease Models, Animal
• Fluorescein (administration & dosage)
• Fluorescein Angiography (methods)
• Fluorescent Dyes (administration & dosage)
• Fundus Oculi
• Inflammation (diagnosis)
• Injections, Intraocular
• Laser Coagulation (adverse effects)
• Leukocytes (pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Retina (pathology, surgery)