MicroRNA-191-5p exerts a tumor suppressive role in renal cell carcinoma.

Abstract

Renal cell carcinoma (RCC) is a common tumor of the urinary system. Previously, miR-191-5p has been reported to be associated with various types of cancer; however, its specific functions in RCC have not been investigated to date. In the present study, the expression of miR-191-5p in the 786-O and ACHN cell lines was detected in vitro by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results of RT-qPCR revealed that miR-191-5p was significantly downregulated in the two cell lines compared with the 293T cell line. miR-191-5p was also significantly downregulated in RCC tissue compared with paired normal tissue. In addition, the effects of miR-191-5p on cell proliferation, migration, invasion and apoptosis were examined by CCK-8, MTT, wound scratch, Transwell and flow cytometry assays. Downregulation of miR-191-5p was observed to promote cell proliferation, migration and invasion, as well as to repress the cell apoptosis of 786-O and ACHN cells. Therefore, the current study suggests that miR-191-5p functions as a tumor suppressor in RCC. Further studies are required to uncover the underlying signaling pathway of miR-191-5p and its potential role as a biomarker for early detection and prognosis prediction, and as a therapeutic target of RCC.

• Authors: Peijie Chen, Xiang Pan, Liwen Zhao, Lu Jin, Canbin Lin, Jing Quan, Tao He, Liang Zhou, Xueling Wu, Yong Wang, Liangchao Ni, Shangqi Yang, Yongqing Lai
• Journal: Experimental and therapeutic medicine (Exp Ther Med) Vol. 15 Issue 2 Pg. 1686-1693 (Feb 2018) ISSN: 1792-0981 [Print] Greece
• PMID: 29434754 (Publication Type: Journal Article)