Transcriptome of Porcine PBMCs over Two Generations Reveals Key Genes and Pathways Associated with Variable Antibody Responses post PRRSV Vaccination.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a virus susceptible to antibody dependent enhancement, causing reproductive failures in sows and preweaning mortality of piglets. Modified-live virus (MLV) vaccines are used to control PRRS in swine herds. However, immunized sows and piglets often generate variable antibody levels. This study aimed to detect significant genes and pathways involved in antibody responsiveness of pregnant sows and their offspring post-PRRSV vaccination. RNA sequencing was conducted on peripheral blood-mononuclear cells (PBMCs), which were isolated from pregnant sows and their piglets with high (HA), median (MA), and low (LA) PRRS antibody levels following vaccination. 401 differentially expressed genes (DEGs) were identified in three comparisons (HA versus MA, HA versus LA, and MA versus LA) of sow PBMCs. Two novel pathways (complement and coagulation cascade pathway; and epithelial cell signaling in H. pylori infection pathway) revealed by DEGs in HA versus LA and MA versus LA were involved in chemotactic and proinflammatory responses. TNF-α, CCL4, and NFKBIA genes displayed the same expression trends in subsequent generation post-PRRS-MLV vaccination. Findings of the study suggest that two pathways and TNF-α, CCL4, and NFKBIA could be considered as key pathways and potential candidate genes for PRRSV vaccine responsiveness, respectively.

• Authors: Ting Yang, Fengxia Zhang, Liwei Zhai, Weiyong He, Zhen Tan, Yangyang Sun, Yuan Wang, Lei Liu, Chao Ning, Weiliang Zhou, Hong Ao, Chuduan Wang, Ying Yu
• Journal: Scientific reports (Sci Rep) Vol. 8 Issue 1 Pg. 2460 (02 06 2018) ISSN: 2045-2322 [Electronic] England
• PMID: 29410429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Chemokine CCL4
• Tumor Necrosis Factor-alpha
• Vaccines, Attenuated
• Viral Vaccines
• NF-KappaB Inhibitor alpha

Topics

• Animals
• Animals, Newborn
• Antibodies, Viral (biosynthesis)
• Chemokine CCL4 (genetics, immunology)
• Complement Pathway, Alternative (drug effects, genetics)
• Female
• Gene Expression Regulation
• Immunity, Humoral (drug effects)
• Immunogenicity, Vaccine
• Leukocytes, Mononuclear (drug effects, immunology, virology)
• NF-KappaB Inhibitor alpha (genetics, immunology)
• Porcine Reproductive and Respiratory Syndrome (genetics, immunology, prevention & control, virology)
• Porcine respiratory and reproductive syndrome virus (growth & development, immunology)
• Pregnancy
• Signal Transduction
• Swine
• Transcriptome (immunology)
• Tumor Necrosis Factor-alpha (genetics, immunology)
• Vaccination
• Vaccines, Attenuated
• Viral Vaccines (administration & dosage)