[Detection of Novel Antigen MLAA-34 Gene Mutation in Acute Monocytic Leukemia and Its Correlation with Efficacy].

Abstract

OBJECTIVE:

To investigate the correlation of all exone mutation in MLAA-34 gene with chemotherapeutic efficacy for leukemia.

METHODS:

The expression level of MLAA-34 gene in 40 patients with AML-M5 and 5 healthy volunteers as control was detected by RT-PCR and its effect on chemotherapeutic efficacy were analyzed by RT-PCR; the effect of MLAA-34 gene mutation on overall survival (OS) and progression-free survival (PFS) of AML-M5 patients was analyzed by sequencing of all 12 exoues in MLAA-34 gene, the correlation between the mutation of prognostic genes important to leukemia and the mutation of MLAA-34 gene was explored.

RESULTS:

The expression level of MLAA-34 gene was significantly up-regulated as compared with that of healthy volunteers, moreover this up-regulation was related with a C59T SNP site located in second exon of MLAA-34 gene, meanswhile this SNP site is affinitive to the well-known mdecular markers of AML, inclinding Fms-like tyrosine kinase (FLT-3) and DNA methyltransferase-3A(DNAMT3A). The AML-M5 patients with high expression of MLAA-34 gene poorly responded to chemotherapy, the AML-M5 patients with MLAA-34 C59T mulation had even more high expression of MLAA-34 gene and significantly short OS and PFS in comparison with those of patients without C59T mutation.

CONCLUSION:

The C59T mutation in MLAA-34 gene is a high risk factor for recurrence of AML, and may be a cadidate target for treatment of AML.

• Authors: Bo Lei, Wang-Gang Zhang, Yin-Xia Chen, Ai-Li He, Xing-Mei Cao, Wan-Hong Zhao, Jian-Li Wang, Jie Liu, Xiao-Rong Ma, Yun Yang, Peng-Yu Zhang, Jing Luo, Xin Meng
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 26 Issue 1 Pg. 97-104 (Feb 2018) ISSN: 1009-2137 [Print] China
• PMID: 29397825 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• DNMT3A protein, human
• DNA Methyltransferase 3A
• fms-Like Tyrosine Kinase 3

Topics

• Antigens, Neoplasm
• DNA Methyltransferase 3A
• Exons
• Humans
• Leukemia, Monocytic, Acute
• Leukemia, Myeloid, Acute
• Mutation
• Prognosis
• fms-Like Tyrosine Kinase 3