Abstract | OBJECTIVES: DESIGN: Animal research study. SETTING: Basic research laboratory in a hospital setting. SUBJECTS: Male Yorkshire-Landrace pigs (50-60 kg). INTERVENTIONS: Pigs underwent 6 hours of intracompartmental pressure elevation by infusing fluid into the anterior compartment of the right hind limb. Carbon monoxide-releasing molecule-3 was administered systemically (2 mg/kg, IV) at fasciotomy, followed by 3-hour reperfusion. MEASUREMENTS AND MAIN RESULTS: Muscle perfusion, inflammation, injury, and apoptosis were assessed in the skeletal muscle. Systemic leukocyte activation was assessed during compartment syndrome and reperfusion. Elevation of hind limb intracompartmental pressure resulted in significant microvascular perfusion deficits (44% ± 1% continuously perfused capillaries in compartment syndrome vs 76% ± 4% in sham; p < 0.001), increased tissue injury ( ethidium bromide/ bisbenzimide of 0.31 ± 0.07 in compartment syndrome vs 0.17 ± 0.03 in sham; p < 0.05), apoptosis (fluorescence in vivo/ bisbenzimide of 0.26 ± 0.06 in compartment syndrome vs 0.13 ± 0.03 in sham; p < 0.05), and systemic leukocyte activation (14.7 relative luminescence units/10 polymorphonuclear leukocytes in compartment syndrome vs 1.0 ± 0.1 in baseline; p < 0.001). Systemic application of carbon monoxide-releasing molecule-3 at fasciotomy increased the number of continuously perfused capillaries (68% ± 3%; p < 0.001), diminished tissue injury ( ethidium bromide/ bisbenzimide of 0.13 ± 0.04; p < 0.05), apoptosis (fluorescence in vivo/ bisbenzimide of 0.12 ± 0.03; p < 0.05), and blocked systemic leukocyte activation (3.9 ± 0.3 relative luminescence unit/10 polymorphonuclear leukocytes; p < 0.001). CONCLUSIONS:
|
Authors | Aurelia Bihari, Gediminas Cepinskas, David Sanders, Abdel-Rahman Lawendy |
Journal | Critical care medicine
(Crit Care Med)
Vol. 46
Issue 5
Pg. e469-e472
(05 2018)
ISSN: 1530-0293 [Electronic] United States |
PMID | 29384781
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Organometallic Compounds
- Tumor Necrosis Factor-alpha
- tricarbonylchloro(glycinato)ruthenium(II)
- Carboxyhemoglobin
|
Topics |
- Animals
- Apoptosis
(drug effects)
- Carboxyhemoglobin
(analysis)
- Compartment Syndromes
(drug therapy, pathology)
- Disease Models, Animal
- Infusions, Intravenous
- Leukocyte Count
- Male
- Muscle, Skeletal
(drug effects, pathology)
- Organometallic Compounds
(administration & dosage, therapeutic use)
- Swine
- Tumor Necrosis Factor-alpha
(blood)
|