Abstract |
Melanoma is one of the most aggressive types of cancer, and its incidence has increased rapidly in the past few decades. In this study, we investigated a novel treatment approach, the use of low-intensity ultrasound (2.3 W/cm2 at 1 MHz)-mediated Optison microbubble (MB) destruction (UMMD) to treat melanoma in a flank tumor model. The effect of UMMD was first evaluated in the melanoma cell line B16 F10 (B16) in vitro and then in mice inoculated with B16 cells. MB+B16 cells were exposed to US in vitro, resulting in significant cell death proportional to duty cycle (R2 = 0.74): approximately 30%, 50%, 80% and 80% cell death at 10%, 30%, 50% and 100% DC respectively. Direct implantation of tumors with MBs, followed by sonication, resulted in retarded tumor growth and improved survival (p = 0.0106). Immunohistochemical analyses confirmed the significant changes in expression of the cell proliferation marker Ki67 (p = 0.037) and a microtubule-associated protein 2 (p = 0.048) after US + MB treatment. These results suggest that UMMD could be used as a possible treatment approach in isolated melanoma and has the potential to translate to clinical trials.
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Authors | Kee W Jang, Dongrim Seol, Lei Ding, Tae-Hong Lim, Joseph A Frank, James A Martin |
Journal | Ultrasound in medicine & biology
(Ultrasound Med Biol)
Vol. 44
Issue 4
Pg. 831-839
(04 2018)
ISSN: 1879-291X [Electronic] England |
PMID | 29361373
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Albumins
- Contrast Media
- FS 069
- Fluorocarbons
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Topics |
- Albumins
(pharmacology)
- Animals
- Cell Death
- Contrast Media
(pharmacology)
- Disease Models, Animal
- Female
- Fluorocarbons
(pharmacology)
- Melanoma
(therapy)
- Mice
- Mice, Inbred C57BL
- Microbubbles
- Ultrasonics
(methods)
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