Ruthenium dendrimers as carriers for anticancer siRNA.

Abstract

Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.

• Authors: Sylwia Michlewska, Maksim Ionov, Marta Maroto-Díaz, Aleksandra Szwed, Aliaksei Ihnatsyeu-Kachan, Svetlana Loznikova, Dzmitry Shcharbin, Marek Maly, Rafael Gomez Ramirez, Francisco Javier de la Mata, Maria Bryszewska
• Journal: Journal of inorganic biochemistry (J Inorg Biochem) Vol. 181 Pg. 18-27 (04 2018) ISSN: 1873-3344 [Electronic] United States
• PMID: 29353086 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Antineoplastic Agents
• Dendrimers
• Intercalating Agents
• RNA, Small Interfering
• Silanes
• carbosilane
• Ruthenium

Topics

• Absorption, Physiological
• Antineoplastic Agents (administration & dosage, chemistry, metabolism)
• Circular Dichroism
• Dendrimers (administration & dosage, chemistry, metabolism)
• Drug Stability
• Dynamic Light Scattering
• HL-60 Cells
• Humans
• Intercalating Agents (administration & dosage, chemistry, metabolism)
• Leukemia, Promyelocytic, Acute (drug therapy, metabolism, pathology)
• Microscopy, Electron, Transmission
• Models, Molecular
• Molecular Conformation
• Molecular Dynamics Simulation
• Molecular Structure
• Particle Size
• RNA Interference
• RNA Stability
• RNA, Small Interfering (administration & dosage, chemistry, metabolism, ultrastructure)
• Ruthenium (administration & dosage, chemistry, metabolism)
• Silanes (chemistry, metabolism)
• Surface Properties