Leishmania tropica isolates from non-healed and healed patients in Iran: A molecular typing and phylogenetic analysis.

Abstract

The precise identification of the parasite species causing leishmaniasis is essential for selecting proper treatment modality. The present study aims to compare the nucleotide variations of the ITS1, 7SL RNA, and Hsp70 sequences between non-healed and healed anthroponotic cutaneous leishmaniasis (ACL) patients in major foci in Iran. A case-control study was carried out from September 2015 to October 2016 in the cities of Kerman and Bam, in the southeast of Iran. Randomly selected skin-scraping lesions of 40 patients (20 non-healed and 20 healed) were examined and the organisms were grown in a culture medium. Promastigotes were collected by centrifugation and kept for further molecular examinations. The extracted DNA was amplified and sequenced. After global sequence alignment with BioEdit software, maximum likelihood phylogenetic analysis was performed in PhyML for typing of Leishmania isolates. Nucleotide composition of each genetic region was also compared between non-healed and healed patients. Our results showed that all isolates belonged to the Leishmania tropica complex, with their genetic composition in the ITS1 region being different among non-healed and healed patients. 7SL RNA and Hsp70 regions were genetically identical between both groups. Variability in nucleotide patterns observed between both groups in the ITS1 region may serve to encourage future research on the function of these polymorphisms and may improve our understanding of the role of parasite genome properties on patients' response to Leishmania treatment. Our results also do not support future use of 7SL RNA and Hsp70 regions of the parasite for comparative genomic analyses.

• Authors: Mehdi Bamorovat, Iraj Sharifi, Mohammad Ali Mohammadi, Sana Eybpoosh, Saeid Nasibi, Mohammad Reza Aflatoonian, Ahmad Khosravi
• Journal: Microbial pathogenesis (Microb Pathog) Vol. 116 Pg. 124-129 (Mar 2018) ISSN: 1096-1208 [Electronic] England
• PMID: 29353004 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• 7SL RNA
• DNA, Protozoan
• DNA, Ribosomal Spacer
• HSP70 Heat-Shock Proteins
• RNA, Small Cytoplasmic
• Signal Recognition Particle

Topics

• Case-Control Studies
• Cities
• Cluster Analysis
• DNA, Protozoan (chemistry, genetics)
• DNA, Ribosomal Spacer (chemistry, genetics)
• Genotype
• HSP70 Heat-Shock Proteins (genetics)
• Humans
• Iran
• Leishmania tropica (classification, genetics, isolation & purification)
• Leishmaniasis, Cutaneous (parasitology)
• Molecular Typing
• Phylogeny
• Polymerase Chain Reaction
• Polymorphism, Genetic
• RNA, Small Cytoplasmic (chemistry, genetics)
• Sequence Analysis, DNA
• Signal Recognition Particle (chemistry, genetics)