Abstract |
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment.
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Authors | Lizenn Delisle, Marine Fuhrmann, Claudie Quéré, Marianna Pauletto, Vianney Pichereau, Fabrice Pernet, Charlotte Corporeau |
Journal | Marine biotechnology (New York, N.Y.)
(Mar Biotechnol (NY))
Vol. 20
Issue 1
Pg. 87-97
(Feb 2018)
ISSN: 1436-2236 [Electronic] United States |
PMID | 29344825
(Publication Type: Journal Article)
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Chemical References |
- Antibodies
- Voltage-Dependent Anion Channels
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Topics |
- Animals
- Antibodies
- Apoptosis
- Crassostrea
(metabolism, virology)
- Herpesviridae
(metabolism)
- Herpesviridae Infections
(metabolism, physiopathology)
- Sequence Analysis, Protein
- Voltage-Dependent Anion Channels
(chemistry, immunology)
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