Abstract | BACKGROUND: METHODS: Male 18-month-old C57BL/6 mice were used. Resveratrol (40 mg/kg) was administered to aged mice for 6 months. We compared histological changes, oxidative stress, and aging-related protein expression in the kidney between the resveratrol-treated group (RSV) and the control group (cont). We performed experiments using small-interfering RNAs (siRNAs) for Nrf2 and SIRT1 in cultured HK2 cells. RESULTS:
Resveratrol improved renal function, proteinuria, histological changes and inflammation in aging mice. Also, expression of Nrf2-HO-1-NOQ-1 signaling and SIRT1-AMPK-PGC-1α signaling was increased in the RSV group. Transfection with Nrf2 and SIRT1 siRNA prevented resveratrol-induced anti-oxidative effect in HK2 cells in media treated with H2O2. CONCLUSIONS: Activation of the Nrf2 and SIRT1 signaling pathways ameliorated oxidative stress and mitochondrial dysfunction. Pharmacological targeting of Nrf2 signaling molecules may reduce the pathologic changes of aging in the kidney.
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Authors | Eun Nim Kim, Ji Hee Lim, Min Young Kim, Tae Hyun Ban, In-Ae Jang, Hye Eun Yoon, Cheol Whee Park, Yoon Sik Chang, Bum Soon Choi |
Journal | Aging
(Aging (Albany NY))
Vol. 10
Issue 1
Pg. 83-99
(01 11 2018)
ISSN: 1945-4589 [Electronic] United States |
PMID | 29326403
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- NF-E2-Related Factor 2
- NFE2L2 protein, human
- RNA, Small Interfering
- HK2 protein, human
- Hexokinase
- SIRT1 protein, human
- Sirtuin 1
- Resveratrol
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Topics |
- Aging
(drug effects)
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(administration & dosage, pharmacology)
- Hexokinase
- Kidney
(drug effects, pathology)
- Male
- Mice, Inbred C57BL
- Mitochondria
(drug effects)
- NF-E2-Related Factor 2
(metabolism)
- Oxidative Stress
(drug effects)
- RNA, Small Interfering
(metabolism)
- Resveratrol
(administration & dosage, pharmacology)
- Signal Transduction
(drug effects)
- Sirtuin 1
(metabolism)
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