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DNA vaccine encoding myristoylated membrane protein (MMP) of rock bream iridovirus (RBIV) induces protective immunity in rock bream (Oplegnathus fasciatus).

Abstract
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6.7 × 105) at 23 °C, with relative percent survival (RPS) of 73.36% and 46.72%, respectively. All of the survivors from the first RBIV infection were strongly protected (100% RPS) from re-infected with RBIV (1.1 × 107) at 100 dpi. In addition, the MMP (ORF008L)-based DNA vaccine significantly induced the gene expression of TLR3 (14.2-fold), MyD88 (11.6-fold), Mx (84.7-fold), ISG15 (8.7-fold), PKR (25.6-fold), MHC class I (13.3-fold), Fas (6.7-fold), Fas ligand (6.7-fold), caspase9 (17.0-fold) and caspase3 (15.3-fold) at 7 days post vaccination in the muscle (vaccine injection site). Our results showed the induction of immune responses and suggest the possibility of developing preventive measures against RBIV using myristoylated membrane protein-based DNA vaccine.
AuthorsMyung-Hwa Jung, Chamilani Nikapitiya, Sung-Ju Jung
JournalVaccine (Vaccine) Vol. 36 Issue 6 Pg. 802-810 (02 01 2018) ISSN: 1873-2518 [Electronic] Netherlands
PMID29325821 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2018 Elsevier Ltd. All rights reserved.
Chemical References
  • Biomarkers
  • Capsid Proteins
  • Cytokines
  • Inflammation Mediators
  • Vaccines, DNA
  • Viral Proteins
Topics
  • Animals
  • Biomarkers
  • Capsid Proteins (genetics, immunology)
  • Cytokines (metabolism)
  • Fish Diseases (genetics, immunology, metabolism, prevention & control)
  • Gene Expression
  • Host-Pathogen Interactions (genetics, immunology)
  • Immunity
  • Immunization
  • Inflammation Mediators
  • Iridovirus (genetics, immunology)
  • Vaccines, DNA (administration & dosage, genetics, immunology)
  • Viral Proteins (genetics, immunology)

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