Abstract | PURPOSE: METHODS: A total of 445 subjects including 200 patients with IBD and 245 controls were allowed in this study. We determined activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx1) and examination their association with the SNPs of respective genes (SOD1 +35A/C, CAT C-262T and GSHPX1 Pro197Leu). RFLP technique was used to determine the selected genes polymorphisms. Antioxidant enzymes activity were evaluated in erythrocyte hemolysate of 23 patients with non-active IBD and 30 healthy participants. RESULTS: The A/C genotype and the C allele frequencies of A/C polymorphism of SOD1 gene were significantly associated with the reduced risk of IBD (OR=0.43; 95% CI 0.23; 0.83). Alike, C/T (OR=0.45; 95% CI= 0.29; 0.70) and T/T genotype (OR=0.43; 95% CI= 0.21; 0.87) of GSHPX1 gene polymorphism diminished the susceptibility to IBD. A significant decrease of CAT (P=0.028) and increase of GPx1 (P=0.025) enzyme activities were seen in IBD patients compared to control. CONCLUSIONS: Our data confirm dysregulated antioxidant capacity in patients suffering from IBD. Both, the SOD1 A/C genotype as well as GSHPX1 C/T and T/T genotypes may be associated with a reduction risk of IBD in the Polish population.
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Authors | Malgorzata Mrowicka, Jerzy Mrowicki, Michal Mik, Radoslaw Wojtczak, Lukasz Dziki, Adam Dziki, Ireneusz Majsterek |
Journal | Oncotarget
(Oncotarget)
Vol. 8
Issue 65
Pg. 109332-109339
(Dec 12 2017)
ISSN: 1949-2553 [Electronic] United States |
PMID | 29312611
(Publication Type: Journal Article)
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