Abstract |
Peroxidasin ( PXDN), an ECM protein with peroxidase activity, is integral to basement membrane consolidation through catalysis of sulfilimine bonds in collagen IV. PXDN is also involved in processes where epithelial-mesenchymal transition (EMT) takes place, namely fibrosis, development and cancer. We therefore investigated whether PXDN is regulated by the EMT-master-regulator, Snai1. During TGF-β1-induced EMT, PXDN expression decreased by up to 47% in two cervical- carcinoma cell lines, with concomitant increases in Snai1 and vimentin, and decrease in E-cadherin. TGF-β1 induced Snai1 binding to the PXDN promoter (as assessed by chromatin immunoprecipitation-PCR) and significantly repressed luciferase reporter gene expression, as did Snai1 overexpression. In summary, our findings show that Snai1 mediates repression of PXDN and consolidate a role for this ECM-modifier during EMT.
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Authors | Boitumelo Nonhlanhla Sitole, Demetra Mavri-Damelin |
Journal | Gene
(Gene)
Vol. 646
Pg. 195-202
(Mar 10 2018)
ISSN: 1879-0038 [Electronic] Netherlands |
PMID | 29305973
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 Elsevier B.V. All rights reserved. |
Chemical References |
- SNAI1 protein, human
- Snail Family Transcription Factors
- Transforming Growth Factor beta1
- PXDN protein, human
- Peroxidases
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Topics |
- Cell Line, Tumor
- Down-Regulation
- Epithelial-Mesenchymal Transition
(drug effects)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- HeLa Cells
- Humans
- Peroxidases
(genetics, metabolism)
- Promoter Regions, Genetic
(drug effects)
- Snail Family Transcription Factors
(metabolism)
- Transforming Growth Factor beta1
(pharmacology)
- Uterine Cervical Neoplasms
(metabolism)
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