Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

Abstract

A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1-5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1-5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity.

• Authors: Hee Jae Shin, Byeoung-Kyu Choi, Phan Thi Hoai Trinh, Hwa-Sun Lee, Jong Soon Kang, Tran Thi Thanh Van, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon Lee
• Journal: Marine drugs (Mar Drugs) Vol. 16 Issue 1 (Jan 05 2018) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 29304006 (Publication Type: Journal Article)

Chemical References

• RANK Ligand
• Sesquiterpenes

Topics

• Animals
• Aspergillus (isolation & purification, metabolism)
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Humans
• Magnetic Resonance Spectroscopy
• Mass Spectrometry
• Osteoclasts (drug effects, metabolism)
• Osteogenesis (drug effects)
• Porifera (microbiology)
• RANK Ligand (metabolism)
• Secondary Metabolism
• Sesquiterpenes (chemistry, isolation & purification, pharmacology)
• Vietnam