T-cell
acute lymphoblastic leukemia (
T-ALL) expands in various bone marrow (BM) sites of the body. We investigated whether different BM sites could differently modulate
T-ALL propagation using in vivo animal models. We observed that mouse and human
T-ALL develop slowly in the BM of tail vertebrae compared with the BM from thorax vertebrae.
T-ALL recovered from tail BM displays lower cell-surface marker expression and decreased metabolism and cell-cycle progression, demonstrating a dormancy phenotype. Functionally, tail-derived
T-ALL exhibit a deficient short-term ex vivo growth and a delayed in vivo propagation. These features are noncell-autonomous because
T-ALL from tail and thorax shares identical genomic abnormalities and functional disparities disappear in vivo and in prolonged in vitro assays. Importantly tail-derived
T-ALL displays higher intrinsic resistance to cell-cycle-related drugs (ie,
vincristine sulfate and
cytarabine). Of note,
T-ALL recovered from gonadal adipose tissues or from cocultures with adipocytes shares metabolic, cell-cycle, and phenotypic or chemoresistance features, with tail-derived
T-ALL suggesting adipocytes may participate in the tail BM imprints on
T-ALL. Altogether these results demonstrate that BM sites differentially orchestrate
T-ALL propagation stamping specific features to leukemic cells such as quiescence and decreased response to cell-cycle-dependent
chemotherapy.