Evaluation of the protective ability of plasma from Japanese individuals against mosquito-borne viral infections.

Abstract

Background:

Most of the Japanese population is seropositive for anti-Japanese encephalitis virus (JEV) antibodies because of previous JEV vaccination or natural infection. Because the virological characteristics of JEV are similar to those of West Nile virus (WNV) and dengue virus (DENV), we hypothesized that anti-JEV antibodies can cross-react with WNV and DENV antigens, leading to protection against infection by these viruses.

Methods:

Using isolated intravenous immunoglobulin (IVIG) from plasma collected in Japan, neutralizing activities against WNV and DENV and antibody-dependent enhancement (ADE) of these viral infections were evaluated using an in vitro assay to determine the potency of immunity against these viruses.

Results:

The prepared IVIG showed considerable neutralizing activity of 2.57 log10 reduction factor against WNV infection but showed little effect against DENV infection. A strong correlation was observed between the neutralizing activity of individual plasma samples against JEV and WNV (ρ=0.768). Moreover, IVIG showed no significant ADE of WNV infection.

Conclusions:

Based on these results, we presume that the Japanese population is generally protected from WNV infection. Furthermore, IVIG prepared from plasma donations from Japanese individuals is expected to be an effective therapeutic agent based on its neutralizing activity against JEV and WNV.

• Authors: Naoya Shinohara, Takashi Owada, Chieko Matsumoto, Shigeharu Uchida, Tadashi Nagai, Masahiro Satake, Kenji Tadokoro
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg) Vol. 111 Issue 9 Pg. 393-401 (09 01 2017) ISSN: 1878-3503 [Electronic] England
• PMID: 29294131 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2017. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Immunoglobulins

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Culicidae (immunology)
• Dengue (prevention & control)
• Dengue Virus (immunology)
• Encephalitis Virus, Japanese (physiology)
• Encephalitis, Japanese (immunology)
• Humans
• Immunoglobulins
• Japan
• Neutralization Tests
• Plasma (immunology)
• West Nile Fever (prevention & control)
• West Nile virus (immunology)