MicroRNA-613 impedes the proliferation and invasion of glioma cells by targeting cyclin-dependent kinase 14.

Abstract

Increasing evidence has suggested that microRNAs (miRNAs) are critical regulators of tumorigenesis. MicroRNA-613 (miR-613) has recently been reported as a novel tumor-related miRNA that plays an important role in multiple cancers. However, the expression and functional significance of miR-613 in glioma remains unclear. In this study, we aimed to investigate the biological function of miR-613 in glioma. We found that miR-613 expression was frequently downregulated in glioma tissues and cell lines compared with normal controls. Overexpression of miR-613 impeded proliferation and colony formation and induced cell cycle arrest in G0/G1 phase, and also inhibited the invasive ability of glioma cells. By contrast, miR-613 inhibition had the opposite effects. Bioinformatic analysis and dual-luciferase reporter assays showed that miR-613 directly targets the 3'-untranslated region of cyclin-dependent kinase 14 (CDK14). Real-time quantitative PCR and Western blot analysis showed that CDK14 expression is negatively regulated by miR-613. In addition, miR-613 expression was inversely correlated with CDK14 expression in clinical glioma tissues. Moreover, overexpression of miR-613 decreased the protein expression of β-catenin and inhibited the activation of Wnt signaling. Importantly, the antitumor effects of miR-613 were significantly reversed by CDK14 overexpression. Overall, our results show that miR-613 inhibits glioma cell proliferation and invasion by downregulating CDK14, suggesting that miR-613 and CDK14 may serve as potential therapeutic targets for the treatment of glioma.

• Authors: Qi Li, Lei Zhou, Maode Wang, Ning Wang, Chuankun Li, Jia Wang, Lei Qi
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 98 Pg. 636-642 (Feb 2018) ISSN: 1950-6007 [Electronic] France
• PMID: 29289838 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Chemical References

• 3' Untranslated Regions
• MicroRNAs
• beta Catenin
• CDK14 protein, human
• Cyclin-Dependent Kinases

Topics

• 3' Untranslated Regions (genetics)
• Cell Cycle Checkpoints (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cyclin-Dependent Kinases (genetics)
• Down-Regulation (genetics)
• G1 Phase (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Glioma (genetics, pathology)
• Humans
• MicroRNAs (genetics)
• Neoplasm Invasiveness (genetics)
• Resting Phase, Cell Cycle (genetics)
• Wnt Signaling Pathway (genetics)
• beta Catenin (genetics)