Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso.

Abstract

Current guidelines for the diagnosis of neurocysticercosis (NCC) recommend the use of the lentil lectin-bound glycoprotein enzyme-linked immunoelectrotransfer blot assay (LLGP-EITB) as the reference standard for serological testing. In response to the drawbacks involved with the use of the LLGP-EITB, a recombinant T24H antigen (rT24H) EITB assay was developed, with promising results. However, the test has yet to be evaluated among individuals from sub-Saharan Africa (SSA). The aim of the present study was to investigate the performance of the rT24H EITB assay for the detection of NCC cases in a panel of serum samples (N = 366, of which 173 patients presented with epileptic seizures and/or severe chronic headaches, and 193 matched manifestation-free participants) collected as part of a large community-based trial in Burkina Faso. A perfect agreement between the rT24H EITB and the native gp24 (and its homodimer, gp42) LLGP-EITB was found (kappa value of 1.0). Furthermore, among patients with the neurological manifestations of interest who underwent a computed tomography scan, the rT24H EITB and native antigen LLGP-EITB had a comparable ability to correctly identify NCC cases with multiple viable (rT24H: sensitivity: 80.0%), single viable (66.7%), and calcified/degenerating cysts only (25.0%), albeit for multiple viable and calcified cysts, the rT24H estimated sensitivity seemed lower, but more uncertain, than previously reported. The rT24H EITB specificity was high (98.2%) and in line with previous studies. This study confirms the value of the recombinant rT24H EITB as an alternative to the native antigen LLGP-EITB for the diagnosis of NCC in a SSA community setting.

• Authors: Veronique Dermauw, Hélène Carabin, Assana Cissé, Athanase Millogo, Zékiba Tarnagda, Rasmané Ganaba, John Noh, Sukwan Handali, Kathleen Breen, Vivian Richter, Rabiou Cissé, Pierre-Marie Preux, Marie-Paule Boncoeur-Martel, Andrea Sylvia Winkler, Anke Van Hul, Pierre Dorny, Sarah Gabriël
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 98 Issue 2 Pg. 565-569 (02 2018) ISSN: 1476-1645 [Electronic] United States
• PMID: 29280427 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Topics

• Adult
• Animals
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Headache (etiology)
• Humans
• Male
• Neurocysticercosis (diagnosis)
• Rural Population (statistics & numerical data)
• Seizures (etiology)
• Surveys and Questionnaires
• Taenia solium (parasitology, pathogenicity)
• Tomography, X-Ray Computed (methods)