Secoisolariciresinol diglucoside inhibits adipogenesis through the AMPK pathway.

Abstract

Flaxseeds are used to treat metabolic diseases such as type 2 diabetes, fatty liver, hyperlipidemia and obesity. Secoisolariciresinol diglucoside (SDG) is a main substance of lignan which belongs to the phytoestrogen family and exists abundantly in flaxseeds. In this study, SDG reduced the body weight and size of adipose tissue, and decreased protein expressions of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer-binding protein α (C/EBPα) in the high fat diet-fed-induced obese mice model. In the vitro study, we examined the anti-adipogenic effect of SDG during differentiation of 3T3-L1 cells into adipocytes. 3T3-L1 preadipocytes were differentiated and treated with various concentrations of SDG. Oil Red O staining was done to measure the quantity of lipid contents. As a result, SDG reduced lipid accumulation and decreased the expressions of adipogenic-related genes such as adipocyte fatty-acid-binding protein 2, adiponectin, and resistin. SDG also decreased the mRNA and protein levels of PPARγ and C/EBPα. Furthermore, phosphorylation levels of AMP-activated protein kinase α (AMPK α) and its upstream activator, liver kinase B1, were significantly increased by SDG in 3T3-L1 cells. These results suggest that SDG inhibits adipogenesis by activating AMPKα, suggesting it could be an attractive therapeutic candidate for the treatment of obesity.

• Authors: JongWook Kang, Jinbong Park, Hye-Lin Kim, Yunu Jung, Dong-Hyun Youn, Seona Lim, Gahee Song, Hyewon Park, Jong Sik Jin, Hyun Jeong Kwak, Jae-Young Um
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 820 Pg. 235-244 (Feb 05 2018) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 29269018 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Butylene Glycols
• Glucosides
• AMP-Activated Protein Kinases
• secoisolariciresinol diglucoside

Topics

• 3T3-L1 Cells
• AMP-Activated Protein Kinases (metabolism)
• Adipocytes (cytology, drug effects)
• Adipogenesis (drug effects)
• Animals
• Butylene Glycols (pharmacology, therapeutic use)
• Cell Survival (drug effects)
• Diet, High-Fat (adverse effects)
• Glucosides (pharmacology, therapeutic use)
• Lipid Metabolism (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• Obesity (chemically induced, drug therapy)
• Signal Transduction (drug effects)